Allelic exchange of unmarked mutations in Mycobacterium tuberculosis.
Allelic exchange of unmarked mutations in Mycobacterium tuberculosis.
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DOI：
10.1007/978-1-59745-232-8_14
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发表时间：
2008
影响因子：
--
通讯作者：
M. Pavelka
中科院分区：
文献类型：
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作者：
M. Pavelka
Mycobacterium tuberculosishas been studied since the 19th century, but genetic manipulation of this organism has only become possible within the last decade of the 20th century. One key methodology is the allelic exchange of unmarked, in-frame deletion mutations and point mutations using counter-selectable suicide plasmids. I describe below the challenges of allelic exchange inM. tuberculosis, the overall mechanism of a suicide vector system, and how it can be used for efficient allelic exchange in this formally intractable organism.
