Ectopic expression of a Ve homolog VvVe gene from Vitis vinifera enhances defense response to Verticillium dahliae infection in tobacco
Ectopic expression of a Ve homolog VvVe gene from Vitis vinifera enhances defense response to Verticillium dahliae infection in tobacco
复制标题
来自葡萄的 Ve 同源物 VvVe 基因的异位表达增强了烟草对大丽黄萎病感染的防御反应
DOI：
10.1016/j.gene.2015.10.068
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发表时间：
2016
期刊：
影响因子：
3.5
通讯作者：
Chang Youhong
中科院分区：
文献类型：
--
作者：
Tang Juan;Lin Jing;Yang Yuwen;Chen Tianzi;Ling Xitie;Zhang Baolong;Chang Youhong
黄萎病是一种土传病害，可对许多重要经济作物的生产造成毁灭性损失。在葡萄属葡萄中鉴定了与黄萎病菌侵染相关的AVe 1同源基因。通过半定量逆转录聚合酶链反应（RT-PCR）鉴定，将“黑风”命名为VvVe。VvVein转基因本氏烟植株的过表达显著增强了对V.大丽花与野生型植物相比。由于VvVe的过度表达，包括水杨酸调节基因病原体相关1（PR 1）而非PR 2、乙烯和茉莉酸调节基因乙烯反应因子1（ERF 1）和脂氧合酶（LOX）在内的防御相关基因的表达显著增加。在VvVe侵染下，转VvVe基因烟草叶片中活性氧、胼胝质和苯丙氨酸解氨酶的积累量均高于野生型烟草。大丽花此外，模拟细胞死亡的过敏反应仅发生在转基因烟草植株中，而不是在野生型中。综上所述，VvVegene基因是一个Ve 1类基因，参与水杨酸、茉莉酸和乙烯等防御信号级联反应，增强对V的防御反应。转基因烟草中大丽花侵染的研究
Verticilliumwilt is a soil borne disease that can cause devastating losses to the production of many economically important crops. AVe1homologous gene responding to Verticillium dahliae infection was identified in Vitis vinifera cv. “HeiFeng” by semi-quantitative reverse transcription polymerase chain reaction and was designated asVvVe. The overexpression ofVvVein transgenic Nicotiana benthamiana plants significantly enhanced the resistance to isolate V991 ofV. dahliaewhen compared with the wild type plants. The expressions of defense-related genes including the salicylic acid regulated gene pathogen-related 1 (PR1) but notPR2, the ethylene- and jasmonic acid-regulated genes ethylene response factor 1 (ERF1) and lipoxygenase (LOX) were significantly increased due to over expression ofVvVe. And greater accumulation of active oxygen, callose and phenylalanine-ammonia lyase were observed in the leaves of transgenicVvVetobacco plants than the wild type when under infection byV. dahliae. Moreover, the hypersensitive response mimicking cell death was exclusively occurred in the transgenicVvVetobacco plants but not in the wild type. Taken together, theVvVegene is aVe1like gene which involves in the signal cascade of salicylic acid, jasmonate, and ethylene defense pathways and enhances defense response toV. dahliaeinfection in the transgenic tobacco.