Expressions of rice sucrose non-fermenting-1 related protein kinase 1 genes are differently regulated during the caryopsis development
Expressions of rice sucrose non-fermenting-1 related protein kinase 1 genes are differently regulated during the caryopsis development
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DOI：
10.1016/j.plaphy.2005.06.004
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发表时间：
2005-07-01
影响因子：
6.5
通讯作者：
Takano, M
中科院分区：
文献类型：
--
作者：
Kanegae, H;Miyoshi, K;Takano, M
水稻蔗糖非发酵1相关蛋白激酶1（SnRK1）家族由三个基因组成，分别命名为OSK1、OSK24和OSK35。为了阐明 OSK 基因的独特功能，我们通过启动子确定了其表达的精确区域：GUS 表达分析以及原位 mRNA 定位实验。首先，我们分离了OSKs每个成员对应的基因组克隆以获得启动子序列。所有 OSK 基因均包含 11 个外显子和 10 个内含子，并且编码区内内含子的位置在所有这些基因中完全保守。使用 OSK 启动子：β-葡萄糖醛酸酶 (OSKP:GUS) 报告基因进行的组织化学分析表明，OSK1P:GUS 和 OSK24P:GUS 在发育中的颖果中的表达模式有很大不同。 OSK1P:GUS在颖果发育过程中的表达几乎局限于维管组织。相比之下，OSK24P：GUS表达在早期的果皮中检测到，随着胚乳细胞的形成而转移到胚乳，并且在开花后15天左右，当细胞胚乳的细胞分裂几乎完成时，GUS染色仅限于糊粉层和胚乳细胞。 OSK24 表达的变化模式与每个组织中淀粉颗粒的出现相关。 OSK24 表达与淀粉颗粒出现之间的类似相关性也在另一个颞库器官（叶鞘的基部）中观察到。这些结果表明 OSK24（水稻 SnRK1b）很可能在库器官的碳水化合物代谢中具有特殊作用。 (c) 2005 年爱思唯尔 SAS。版权所有。
The rice sucrose non-fermenting-1 related protein kinase 1 (SnRK1) family consists of three genes, which were named OSK1, OSK24 and OSK35. In order to elucidate the distinct functions of OSK genes, we identified precise regions for their expression by the promoter: GUS expression analyses as well as in situ mRNA localization experiments. At first, we isolated genomic clones corresponding to each member of OSKs in order to obtain the promoter sequences. All OSK genes house 11 exons and 10 introns and the positions of introns within the coding regions are fully conserved in all these genes. Histochemical analyses using OSK promoter: beta-glucronidase (OSKP:GUS) reporter genes showed that expression patterns of OSK1P:GUS and OSK24P:GUS were quite different in the developing caryopsis. The expression of OSK1P:GUS was nearly restricted in the vascular tissues during the caryopsis development. In contrast, the OSK24P:GUS expression was detected in the pericarp, at the early stage with a shift to the endosperm as the enclosperm cells were formed, and GUS staining was confined to both aleurone layer and endosperm cells around 15 days after flowering, when cell division of cellular endosperm were almost finished. The shifting pattern of the OSK24 expression was correlated with the appearance of starch granules in each tissue. Similar correlation between OSK24 expression and emergence of starch granules was also observed at another temporal sink organ, the basal part of leaf sheath. These results suggest that OSK24 (rice SnRK1b) most probably have a special role in carbohydrate metabolism of the sink organs. (c) 2005 Elsevier SAS. All rights reserved.