Forensic analysis of mesembrine alkaloids in Sceletium tortuosum by nonaqueous capillary electrophoresis mass spectrometry
Forensic analysis of mesembrine alkaloids in Sceletium tortuosum by nonaqueous capillary electrophoresis mass spectrometry
复制标题
非水毛细管电泳质谱法对 Sceletium tortuosum 中的 mesembrine 生物碱进行法医分析
作者：
Jörg Roscher;T. Posch;M. Pütz;C. Huhn
合法和非法药物的消费遵循与当前食品消费趋势相当的有机趋势。因此，对这些药物的研究对表征植物和药物制剂的有效成分具有重要意义。本文提出了一种新的非水毛细管电泳-质谱联用技术（NACE - MS），该技术是分离复杂基质中复杂生物碱混合物的有力工具，可用于分析龙参及其制剂Kanna。结果表明，该方法适用于药材中生物碱成分的测定和相对定量分析。它被证明是有价值的分离大量的等压化合物，最可能包括非对映体，双键异构体，和进一步的结构密切相关的化合物。对来自不同供应商的植物样品、自发酵样品和准备消费的产品进行了比较。与迄今为止文献中提出的其他方法相比，所获得的高分离功率可以更好地描述植物样品以及卡纳制剂的化学型差异。因此，使用NACE - MS可以从一个新的角度来研究剑鞘物种的生物碱谱。
The consumption of legal and illegal drugs follows an organic trend comparable to the current trend in food consumption. The investigation of such drugs is therefore of interest to characterize the active ingredients of plants and drug preparations. A new method of nonaqueous capillary electrophoresis coupled to mass spectrometry (NACE‐MS) as a powerful tool for the separation of complex alkaloid mixtures in difficult matrices is presented in this study for the analysis of samples of Sceletium tortuosum and drug products called Kanna made thereof. The method was found to be suitable for the investigation of the alkaloid composition and relative quantification of the ingredients. It proved of value to separate a large number of isobaric compounds, most probably including diastereomers, double‐bond isomers, and further structurally closely related compounds. A comparison of plant samples from different vendors, self‐fermented samples, and products ready for consumption was made. The high separation power obtained allowed a better description of the chemotypic differences of plant samples as well as Kanna preparations compared to other methods presented in the literature so far. Thus, the use of the NACE‐MS enables a new perspective on the alkaloid profile of Sceletium species.