Involvement of BcVeA and BcVelB in regulating conidiation, pigmentation and virulence in Botrytis cinerea
Involvement of BcVeA and BcVelB in regulating conidiation, pigmentation and virulence in Botrytis cinerea
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BcVeA 和 BcVelB 参与调节灰霉病分生孢子形成、色素沉着和毒力
DOI：
10.1016/j.fgb.2012.10.003
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发表时间：
2013-01-01
影响因子：
3
通讯作者：
Ma, Zhonghua
中科院分区：
文献类型：
--
作者：
Yang, Qianqian;Chen, Yanfen;Ma, Zhonghua
异源三聚天鹅绒复合物VeA/VelB/LaeA参与构巢曲霉中多种细胞过程的调节。本研究对灰葡萄孢菌中两个绒状基因BcVEA和BcVELB的功能进行了研究。BcVEA和BcVELB缺失突变体的形态学特征表明，BcVEA和BcVELB的缺失导致产孢和黑色素生物合成增加。BcVEA和BcVELB缺失突变体也表现出对氧化应激的敏感性增加。致病性试验表明，BcVeA和BcVel B对B的完全毒力是必需的。灰叶酵母双杂交试验显示BcVeA与BcVelB相互作用。这项研究的结果表明，BcVeA和BcVelB协调类似的过程中的真菌发育，氧化应激反应，和毒性的调节B。灰叶(C)2012 Elsevier Inc. All rights reserved.
The heterotrimeric velvet complex VeA/VelB/LaeA is involved in the regulation of diverse cellular processes in Aspergillus nidulans. In this work, we investigated functions of two velvet-like genes BcVEA and BcVELB in Botrytis cinerea. Morphological characterization of BcVEA and BcVELB deletion mutants showed that the deletion of BcVEA and BcVELB led to increased conidiation and melanin biosynthesis. BcVEA and BcVELB deletion mutants also showed increased sensitivity to oxidative stress. Pathogenicity assays revealed that both BcVeA and BcVelB were essential for full virulence of B. cinerea. Yeast two-hybrid assay displayed the interaction of BcVeA with BcVelB. Results of this study indicate that BcVeA and BcVelB coordinate similar processes in the regulation of fungal development, oxidative stress response, and virulence in B. cinerea. (C) 2012 Elsevier Inc. All rights reserved.