CAPS1 is involved in hippocampal synaptic plasticity and hippocampus-associated learning.
CAPS1 is involved in hippocampal synaptic plasticity and hippocampus-associated learning.
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CAPS1参与海马突触可塑性和海马相关学习。
DOI：
10.1038/s41598-021-88009-w
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发表时间：
2021-04-21
影响因子：
4.6
通讯作者：
Furuichi T
中科院分区：
文献类型：
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作者：
Ishii C;Shibano N;Yamazaki M;Arima T;Kato Y;Ishii Y;Shinoda Y;Fukazawa Y;Sadakata T;Sano Y;Furuichi T
钙依赖性分泌激活蛋白1（CAPS 1）是囊泡胞吐的关键分子，可能在启动步骤中。然而，CAPS 1在突触可塑性和脑功能中的作用是难以捉摸的。在此，我们表明，突触可塑性和学习行为受损前脑和/或海马特异性Caps 1条件性敲除（cKO）小鼠的分子，生理和行为分析的手段。新生Caps 1 cKO小鼠显示海马CA 3区停靠囊泡数量减少，其他主要胞吐相关分子的分布没有检测到变化。此外，长时程增强（LTP）在CA 1和CA 3区域分别部分和严重受损。重复高频刺激可使CA 1 LTP增强，而CA 3 LTP完全消失。因此，在腺相关病毒（AAV）感染介导的出生后Caps 1 cKO小鼠中，海马区相关学习严重受损。总的来说，我们的研究结果表明，CAPS 1是一个关键的蛋白质参与海马突触释放和可塑性，这是至关重要的海马相关的学习的细胞机制。
Calcium-dependent activator protein for secretion 1 (CAPS1) is a key molecule in vesicular exocytosis, probably in the priming step. However, CAPS1’s role in synaptic plasticity and brain function is elusive. Herein, we showed that synaptic plasticity and learning behavior were impaired in forebrain and/or hippocampus-specific Caps1 conditional knockout (cKO) mice by means of molecular, physiological, and behavioral analyses. Neonatal Caps1 cKO mice showed a decrease in the number of docked vesicles in the hippocampal CA3 region, with no detectable changes in the distribution of other major exocytosis-related molecules. Additionally, long-term potentiation (LTP) was partially and severely impaired in the CA1 and CA3 regions, respectively. CA1 LTP was reinforced by repeated high-frequency stimuli, whereas CA3 LTP was completely abolished. Accordingly, hippocampus-associated learning was severely impaired in adeno-associated virus (AAV) infection-mediated postnatal Caps1 cKO mice. Collectively, our findings suggest that CAPS1 is a key protein involved in the cellular mechanisms underlying hippocampal synaptic release and plasticity, which is crucial for hippocampus-associated learning.
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影响因子：
4.6
作者：
Shinoda Y;Ishii C;Fukazawa Y;Sadakata T;Ishii Y;Sano Y;Iwasato T;Itohara S;Furuichi T
通讯作者：
Furuichi T
DOI：
10.1073/pnas.1012220108
发表时间：
2011-01-04
影响因子：
11.1
作者：
Shinoda, Yo;Sadakata, Tetsushi;Furuichi, Teiichi
通讯作者：
Furuichi, Teiichi
影响因子：
64.8
作者：
Iwasato, T;Datwani, A;Itohara, S
通讯作者：
Itohara, S
影响因子：
7.2
作者：
Luescher, Christian;Malenka, Robert C.
通讯作者：
Malenka, Robert C.
影响因子：
16.8
作者：
通讯作者：
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