Factors influencing the growth of alveolar type II epithelial cells isolated from rat lungs.
Factors influencing the growth of alveolar type II epithelial cells isolated from rat lungs.
复制标题
影响从大鼠肺中分离的肺泡 II 型上皮细胞生长的因素。
DOI：
10.1016/0014-4827(82)90383-4
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发表时间：
1982
影响因子：
3.7
通讯作者：
Holley,RW
中科院分区：
文献类型：
--
作者：
Messmer,TO;Armour,R;Holley,RW
已经建立了分离的肺泡II型细胞的原代培养物。在适当的条件下，这些上皮细胞可以传代培养至少9次。使用标准测定程序，可以研究生长因子或抑制剂的作用。肺泡II型细胞在组织培养中对血清和生长因子均显示出显著的反应。表皮生长因子（EGF）或人尿均可增加胸苷掺入（分别为2倍和10倍）。尿液中的生长因子似乎与尿抑胃素（人EGF）不同。对尿液的反应比单独对饱和浓度小鼠EGF的反应大几倍。将小鼠EGF添加到尿液中不会增加尿液的活性。肺泡II型细胞对尿中生长因子的反应期仅限于细胞的早期传代。肺泡II型上皮细胞在培养中产生生长抑制剂。在存在或不存在血清的情况下，生长培养基中产生抑制剂。浓缩的抑制剂，虽然非常不稳定，给出了高达50%的抑制胸苷掺入时，稀疏或拥挤的肺泡II型细胞进行测定。
Primary cultures of isolated alveolar type II cells have been established. Under appropriate conditions, these epithelial cells can be subcultured at least nine times. Using standard assay procedures, effects of growth factors or inhibitors can be studied. The alveolar type II cells show a marked response to both serum and growth factors in tissue culture. Either epidermal growth factor (EGF) or human urine gives an increase in thymidine incorporation (2-fold and 10-fold, respectively). The growth factor(s) in urine appears to be different from urogastrone (human EGF). The response to urine is several-fold greater than the response to a saturating concentration of mouse EGF alone. Mouse EGF added to urine does not increase the activity of urine. The period during which the alveolar type II cells respond to the growth factor(s) in urine is limited to early passages of the cells. Alveolar type II epithelial cells produce growth inhibitors in culture. Inhibitors are produced in the growth medium in either the presence or absence of serum. The concentrated inhibitor, although very unstable, gives up to a 50% inhibition of thymidine incorporation when assayed on sparse or crowded alveolar type II cells.