Radiation-induced sterility for pupal and adult stages of the malaria mosquito Anopheles arabiensis
Radiation-induced sterility for pupal and adult stages of the malaria mosquito Anopheles arabiensis
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DOI：
10.1186/1475-2875-5-41
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发表时间：
2006-05-15
期刊：
影响因子：
3
通讯作者：
Knols, BGJ
中科院分区：
文献类型：
--
作者：
Helinski, MEH;Parker, AG;Knols, BGJ
背景资料：在昆虫不育技术（SIT）的背景下，对阿拉伯按蚊（双翅目：蚊科）辐射诱导不育进行了研究。雄蚊在蛹期或成虫期暴露于γ射线，并测定剂量-不育曲线。方法：蛹在羽化前不久（22 - 26小时）照射，成虫在羽化后< 24小时照射。试验剂量范围为0 - 100戈伊。研究了辐射对成虫羽化、雄虫存活、诱导不育和授精能力的影响。使用对照组的独立t检验分析羽化和授精数据。对授精率、授精剂量与授精效果进行相关分析。使用Kaplan-Meier生存分析对雄性动物生存进行分析。最后，计算出的剩余生育力值进行反正态转换和线性回归analysesperformed.Results：辐照的pueridae，为所有剂量的测试，对成虫出现没有影响。受照雄性的存活曲线与未受照雄性相似，甚至略高于未受照雄性。总体而言，成年人似乎对辐照的敏感性略高，但未观察到个体剂量的显著差异。在蛹期，授精与剂量呈显著负相关，但相关系数小于总变异的25%。文献回顾表明，一个。阿拉伯按蚊比其他按蚊更耐辐射。结论：SIT计划中雄性昆虫释放的最佳剂量取决于它们的不育水平和竞争力。使用半灭菌剂量，以产生更有竞争力的昆虫进行了讨论。大规模照射蚊子最方便的发育阶段是蛹期，但与成虫照射相比，蛹照射在最高剂量下的授精率较低。在本研究的基础上，确定了包括半灭菌剂量在内的合适剂量范围，以启动在两个发育阶段接受辐射的雄性的竞争力实验。
Background: In the context of the Sterile Insect Technique ( SIT), radiation-induced sterility in the malaria mosquito Anopheles arabiensis Patton ( Diptera: Culicidae) was studied. Male mosquitoes were exposed to gamma rays in the pupal or adult stage and dose-sterility curves were determined.Methods: Pupae were irradiated shortly before emergence ( at 22 - 26 hrs of age), and adults < 24 hrs post emergence. Doses tested ranged between 0 and 100 Gy. The effects of irradiation on adult emergence, male survival, induced sterility and insemination capability were evaluated. Emergence and insemination data were analysed using independent t-tests against the control. Correlation analyses were performed for insemination rate and dose and insemination and fecundity. Male survival was analysed using Kaplan-Meier survival analyses. Finally, the calculated residual fertility values were inverse-normal transformed and linear regression analyses performed.Results: Irradiation of pupae, for all doses tested, had no effect on adult emergence. Survival curves of males irradiated as pupae or adults were similar or even slightly higher than non-irradiated males. Overall, adults appeared to be slightly more susceptible to irradiation, although no significant differences for individual doses were observed. In the pupal stage, a significant negative correlation was found between insemination and dose, but the correlation-coefficient was associated with less than 25% of the total variation. A review of the literature indicated that An. arabiensis is more radiation resistant than other anopheline mosquitoes.Conclusion: The optimal dose for male insects to be released in an SIT programme depends on their level of sterility and competitiveness. The use of semi-sterilizing doses to produce more competitive insects is discussed. The most convenient developmental stage for mosquito irradiation on a mass-scale are pupae, but pupal irradiation resulted in a lower insemination rate at the highest dose compared to adult irradiation. On the basis of this study, a suitable dose range that includes semi-sterilizing doses is identified to initiate competitiveness experiments for males irradiated at both developmental stages.