S-(4-Nitrophenacyl)glutathione is a specific substrate for glutathione transferase omega 1-1
S-(4-Nitrophenacyl)glutathione is a specific substrate for glutathione transferase omega 1-1
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DOI：
10.1016/j.ab.2007.09.029
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发表时间：
2008-03-01
影响因子：
2.9
通讯作者：
Anders, M. W.
中科院分区：
文献类型：
--
作者：
Board, Philip G.;Coggan, Marjorie;Anders, M. W.
谷胱甘肽转移酶omega 1-1(GSTO1-1)催化砷的生物转化，并被认为是影响阿尔茨海默病发病年龄和白介素1β(IL-1β)翻译后激活的一个因素。由于缺乏对含有其他GST和具有类似催化特性的其他酶的组织样本中GSTO1-1活性的特异性检测，对GSTO1-1变体的生物学作用的研究一直受到阻碍。以前的研究(P.G.Board和M.W.Anders，Chem.Toxicol资源。20(2007)149-154)的研究表明，GSTO1-1催化S-(苯乙基)谷胱甘肽还原为苯乙酮。合成了一种新的底物--S-(4-硝基苯乙酰)谷胱甘肽(4NPG)，它与谷胱甘肽转移酶超家族成员GSTO2-1的转化率很高，而与GSTO2-2和其他成员的转化率很低。以4NPG为底物，用分光光度法测定了几种人乳腺癌细胞系及小鼠肝、脑组织中GSTO1-1的活性。(C)2007 Elsevier Inc.保留所有权利。
Glutathione transferase omega 1-1 (GSTO1-1) catalyzes the biotransformation of arsenic and is implicated as a factor influencing the age-at-onset of Alzheimer's disease and the posttranslational activation of interleukin 1 beta (IL-1 beta). Investigation of the biological role of GSTO1-1 variants has been hampered by the lack of a specific assay for GSTO1-1 activity in tissue samples that contain other GSTs and other enzymes with similar catalytic specificities. Previous studies (P. G. Board and M. W. Anders, Chem. Res. Toxicol. 20 (2007) 149-154) have shown that GSTO1-1 catalyzes the reduction of S-(phenacyl)glutathiones to acetophenones. A new substrate, S-(4-nitrophenacyl)glutathione (4NPG), has been prepared and found to have a high turnover with GSTO1-1 but negligible activity with GSTO2-2 and other members of the glutathione transferase superfamily. A spectrophotometric assay with 4NPG as a substrate has been used to determine GSTO1-1 activity in several human breast cancer cell lines and in mouse liver and brain tissues. (c) 2007 Elsevier Inc. All rights reserved.