Volumetric stimulated Raman scattering imaging of cleared tissues towards three-dimensional chemical histopathology
Volumetric stimulated Raman scattering imaging of cleared tissues towards three-dimensional chemical histopathology
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DOI：
10.1364/boe.10.004329
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发表时间：
2019-08-01
影响因子：
3.4
通讯作者：
Cheng, Ji-Xin
中科院分区：
文献类型：
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作者：
Li, Junjie;Lin, Peng;Cheng, Ji-Xin
基于薄组织切片的组织学自一百多年前以来一直被用作疾病诊断的金标准。然而，二维切片上的组织病理学评价由于取样有限而存在较大差异。为了提高诊断准确性，三维（3D）组织学是通过连续切片，染色，成像和重建的个别切片，这是非常耗时和劳动密集型。我们开发了一种体积受激拉曼散射（SRS）成像方法，它提供了组织学的三维背景下，而不需要染色染料的信息。使用小分子清除剂甲酰胺，我们在30分钟内进行了组织清除，并在高度分散的组织中实现了高达500 μ m的成像深度，包括脑、肾、肝和肺。通过双色SRS成像方案，我们在透明脑组织切片中获得了组织学样图像。我们的方法有潜力的三维组织病理学，以提高组织病理学检查的准确性。(C)根据OSA开放获取出版协议的条款，2019年美国光学学会
Thin tissue slice based histology has been used as a gold standard for disease diagnosis since over a hundred years ago. However, histopathological evaluation on two-dimensional slides suffers from large variations duc to limited sampling. To improve the diagnostic accuracy, three-dimensional (3D) histology is performed through serial sectioning, staining, imaging and reconstruction of individual slices, which is highly time-consuming and labor intensive. We developed a volumetric stimulated Raman scattering (SRS) imaging method, which provides histology-like information in 3D context without the need for staining with dyes. Using a small molecule clearing agent, formamide, we performed tissue clearing Within 30 min and achieved an imaging depth up to 500 pm in highly scattered tissues, including brain, kidney; liver and lung. Through a two-color SRS imaging scheme, we obtained histology-like images in cleared brain tissue slices. Our method has the potential for 3D tissue histopathology to improve the accuracy of histopathological examination. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement