Salt tolerance of barley induced by the root endophyte Piriformospora indica is associated with a strong increase in antioxidants
Salt tolerance of barley induced by the root endophyte Piriformospora indica is associated with a strong increase in antioxidants
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DOI：
10.1111/j.1469-8137.2008.02583.x
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发表时间：
2008-01-01
期刊：
影响因子：
9.4
通讯作者：
Skoczowski, Andrzej
中科院分区：
文献类型：
--
作者：
Baltruschat, Helmut;Fodor, Jozsef;Skoczowski, Andrzej
在许多植物中，根内生担子菌Piriformospora indica已被证明具有增强生物胁迫抗性和非生物胁迫耐受性的作用。以抗氧化剂为重点，研究了大麦（Hordeum vulgare）耐盐性的生化机制。评估盐胁迫的生理指标，如代谢活性、脂肪酸组成、脂质过氧化、抗坏血酸浓度以及过氧化氢酶、抗坏血酸过氧化物酶、脱氢抗坏血酸还原酶、单脱氢抗坏血酸还原酶和谷胱甘肽还原酶的活性。在盐敏感大麦品种英格丽德（Ingrid）中，P. indica的根定殖促进了植株生长，减弱了nacl诱导的叶片脂质过氧化、代谢性热外排和脂肪酸去饱和。在盐胁迫条件下，内生菌显著提高了大麦根系抗坏血酸含量和抗氧化酶活性。同样，在耐盐大麦品种California Mariout的nacl处理根中，抗氧化系统的持续上调被证明，与籼稻的植物定植无关。这些发现表明抗氧化剂可能在遗传和内生菌介导的植物耐盐性中发挥作用。
The root endophytic basidiomycete Piriformospora indica has been shown to increase resistance against biotic stress and tolerance to abiotic stress in many plants.Biochemical mechanisms underlying P. indica-mediated salt tolerance were studied in barley (Hordeum vulgare) with special focus on antioxidants. Physiological markers for salt stress, such as metabolic activity, fatty acid composition, lipid peroxidation, ascorbate concentration and activities of catalase, ascorbate peroxidase, dehydroascorbate reductase, monodehydroascorbate reductase and glutathione reductase enzymes were assessed.Root colonization by P. indica increased plant growth and attenuated the NaCl-induced lipid peroxidation, metabolic heat efflux and fatty acid desaturation in leaves of the salt-sensitive barley cultivar Ingrid. The endophyte significantly elevated the amount of ascorbic acid and increased the activities of antioxidant enzymes in barley roots under salt stress conditions. Likewise, a sustained up-regulation of the antioxidative system was demonstrated in NaCl-treated roots of the salt-tolerant barley cultivar California Mariout, irrespective of plant colonization by P. indica.These findings suggest that antioxidants might play a role in both inherited and endophyte-mediated plant tolerance to salinity.