Polo-like kinase Cdc5 drives exit from pachytene during budding yeast meiosis
Polo-like kinase Cdc5 drives exit from pachytene during budding yeast meiosis
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DOI：
10.1101/gad.1711408
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发表时间：
2008-10-01
影响因子：
10.5
通讯作者：
Lichten, Michael
中科院分区：
文献类型：
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作者：
Sourirajan, Anuradha;Lichten, Michael
在芽殖酵母中，退出减数分裂的粗线期阶段需要减数分裂中期转录因子Ndt80，其促进类似于200个基因的表达。Ndt80是Polo样激酶（PLK，Cdc5）和细胞周期蛋白依赖性激酶（CDK）的减数分裂功能所必需的，这两种细胞周期激酶先前与粗线期退出有关。我们发现，持续的CDK活性是伴随着退出粗线期的两个事件：交叉形成和联会复合体的崩溃。相比之下，CDC5在CD80 Delta突变体中的表达有效地促进了这两个事件。因此，Cdc5是减数分裂进程中这一关键步骤所需的Ndt80转录组的唯一成员。
In budding yeast, exit from the pachytene stage of meiosis requires the mid-meiosis transcription factor Ndt80, which promotes expression of similar to 200 genes. Ndt80 is required for meiotic function of polo-like kinase (PLK, Cdc5) and cyclin-dependent kinase (CDK), two cell cycle kinases previously implicated in pachytene exit. We show that ongoing CDK activity is dispensable for two events that accompany exit from pachytene: crossover formation and synaptonemal complex breakdown. In contrast, CDC5 expression in ndt80 Delta mutants efficiently promotes both events. Thus, Cdc5 is the only member of the Ndt80 transcriptome required for this critical step in meiotic progression.