GLUTAMATE INDUCES CALCIUM WAVES IN CULTURED ASTROCYTES - LONG-RANGE GLIAL SIGNALING
GLUTAMATE INDUCES CALCIUM WAVES IN CULTURED ASTROCYTES - LONG-RANGE GLIAL SIGNALING
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DOI：
10.1126/science.1967852
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发表时间：
1990-01-26
期刊：
影响因子：
56.9
通讯作者：
SMITH, SJ
中科院分区：
文献类型：
--
作者：
CORNELLBELL, AH;FINKBEINER, SM;SMITH, SJ
星形胶质细胞具有谷氨酸敏感离子通道的发现暗示了这些细胞以前未被认识到的信号作用。现在有报道称，培养的海马星形胶质细胞对谷氨酸的反应是胞质游离钙的迅速和振荡性升高，通过荧光钙指示剂fluo-3可见。两种类型的谷氨酸受体，一种倾向于半胱甘酸盐并从细胞内释放钙，另一种倾向于盐酸盐并促进表面膜钙的流入。此外，在融合培养中，谷氨酸诱导的细胞质游离钙的增加经常以波的形式在单个星形胶质细胞的细胞质内和相邻星形胶质细胞之间传播。这些钙的传播波提示星形胶质细胞网络可能构成大脑内的远程信号系统。
The finding that astrocytes prossess glutamate-sensitive ion channels hinted at a previously unrecognized signaling role for these cells. Now it is reported that cultured hippocampal astrocytes can respond to glutamate with a prompt and oscillatory elevation of cytoplasmic free calcium, visible through use of the fluorescent calcium indicator fluo-3. Two types of glutamate receptor.sbd.one preferring quisqalate and releasing calcium from intracellular stores and the preferring kainate and promoting surface-membrane calcium influx.sbd.appear to be involved. Moreover, glutamate-induced increases in cytoplasmic free calcium frequently propagate as waves within the cytoplasm of individual astrocytes and between adjacent astrocytes in confluent cultures. These propagating waves of calcium suggest that networks of astrocytes may constitute a long-range signaling system within the brain.