Identification of ganglio-N-tetraosylceramide as a new cell surface marker for murine natural killer (NK) cells.
Identification of ganglio-N-tetraosylceramide as a new cell surface marker for murine natural killer (NK) cells.
复制标题
鉴定神经节-N-四糖神经酰胺作为小鼠自然杀伤 (NK) 细胞的新细胞表面标记。
作者：
W. Young;S. Hakomori;J. Durdik;C. Henney
通过将效应细胞与抗神经节-N-四糖基神经酰胺（抗去唾液酸 GM1）和 C 预孵育，BCG 诱导的小鼠腹膜渗出细胞的 NK 细胞活性被消除，但与其他抗糖脂抗体、抗神经节苷脂 GM1、抗球苷和抗神经节-N-三糖神经酰胺（抗去唾液酸 GM2）一起预孵育不会被消除。相比之下，同种免疫T细胞的细胞毒活性不受抗脱唾液酸GM1抗血清处理的影响。这些发现表明，asialo GM1 展示可能是 NK 细胞群的特征，有助于分离该细胞毒性细胞群。
The BCG-induced NK cell activity of murine peritoneal exudate cells was abolished by preincubation of effector cells with anti-ganglio-N-tetraosylceramide (anti-asialo GM1) and C but not with other anti-glycolipid antibodies, anti-ganglioside GM1, anti-globoside, and anti-ganglio-N-triosylceramide (anti-asialo GM2). In contrast, the cytotoxic activity of alloimmune T cells was not affected by treatment with anti-asialo GM1 antisera. These findings suggest that asialo GM1 display may be characteristic of NK cell populations and aid in the isolation of this population of cytotoxic cells.