Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire
Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire
复制标题
DOI：
10.4049/jimmunol.166.11.6477
复制
发表时间：
2001-06-01
影响因子：
4.4
通讯作者：
Butcher, EC
中科院分区：
文献类型：
--
作者：
Campbell, JJ;Qin, SX;Butcher, EC
CD56是一种与神经细胞黏附分子密切相关的黏附分子，是几种独特的外周血淋巴细胞（PBLs）群体的免疫表型标志物。尽管CD56（+）细胞来源于多种淋巴细胞谱系，但它们在免疫监视和抗肿瘤反应中具有共同作用。我们研究了来自人外周血的三种不同的CD56（+）群体的趋化因子受体表达模式和功能性迁移反应。发现自然杀伤T细胞（NK - T细胞）与自然杀伤细胞（NK细胞）有很大差异，CD16（+）NK细胞与CD16（-）NK细胞也不同。CD16（+）NK细胞是对白细胞介素 - 8（IL - 8）和分形趋化因子（fractalkine）起主要反应的群体，而NK - T细胞是对CCR5配体巨噬细胞炎性蛋白 - 1β起主要反应的群体。CD16（-）NK细胞是唯一均匀表达通过高内皮微静脉归巢到次级淋巴器官所必需的运输分子的CD56（+）群体。这些发现描述了一个多样的细胞群体，它们可能具有彼此完全不同且与其他淋巴细胞类型也不同的运输模式。
CD56, an adhesion molecule closely related to neual cell adhesion molecule, is an immunophenotypic marker for several unique populations of PBLs. Although CD56(+) cells derive from multiple lymphocyte lineages, they share a role in immunosurveillance and antitumor responses. We have studied the chemokine receptor expression patterns and functional migratory responses of three distinct CD56(+) populations from human peripheral blood. NK-T cells were found to differ greatly from NK cells, and CD16(+) NK cells from CD16(-) NK cells. CD16(+) NK cells were the predominant population responding to IL-8 and fractalkine, whereas NK-T cells were the predominant population responding to the CCR5 ligand macrophage-inflammatory protein-1 beta. CD16(-) NK cells were the only CD56(+) population that uniformly expressed trafficking molecules necessary for homing into secondary lymphoid organs through high endothelial venule. These findings describe a diverse population of cells that may have trafficking patterns entirely different from each other, and from other lymphocyte types.