Screening of pesticide residues in soil and water samples from agricultural settings
Screening of pesticide residues in soil and water samples from agricultural settings
复制标题
DOI：
10.1186/1475-2875-5-22
复制
发表时间：
2006-03-24
期刊：
影响因子：
3
通讯作者：
Kinde-Gazard, Dorothee A.
中科院分区：
文献类型：
--
作者：
Akogbeto, Martin C.;Djouaka, Rousseau F.;Kinde-Gazard, Dorothee A.
背景资料：农业做法在疟疾病媒选择杀虫剂抗药性方面的作用迄今为止一直被假设为没有明确的证据。许多蚊子物种，特别是冈比亚按蚊，在农业环境周围的繁殖地产卵。由于农业活动，土壤和水中可能存在杀虫剂残留物，这些残留物对各种蚊子种群的幼虫阶段产生选择压力。为了证实这一假设，在贝宁共和国进行了一项研究，以评估环境的危害，可以产生大量使用农药在农业settings.Methods：缺乏HPLC机直接定量样品中的杀虫剂残留，这项调查是使用间接生物测定集中在研究的因素抑制蚊子幼虫的正常生长繁殖场所。监测其发育速度以及饲养的产量。在繁殖地，冈比亚幼虫用在已知受农药压力影响的农业地区收集的水和土壤样本复原。两种菌株。在该间接生物测定中使用冈比亚的拟除虫菊酯敏感性基苏穆品系和抗性Ladji品系。在这种方法中的关键方法是基于比较的幼虫在测试和控制繁殖场所的生长，测试样品已收集从两个蔬菜farm.Results：获得的结果清楚地显示存在的抑制因素的测试样品。在对照样品中观察到幼虫的正常生长。在模拟繁殖场所，使用几克的土壤样品从两个蔬菜农场在恒定的杀虫剂处理（测试样品），一个穷人的孵化率的按蚊卵加上延迟的幼虫生长和成蚊的产量低，从孵化的卵，noted.Conclusion：有毒因素抑制按蚊卵孵化和幼虫的生长可能是农药残留的农业实践。本间接测定中使用的样品已储存在实验室中，并将采用HPLC技术进行分析，以确认本研究的假设，并确定在农药压力下农业环境中土壤和水样品中发现的各种最终产品。
Background: The role of agricultural practices in the selection of insecticide resistance in malaria vectors has so far been hypothesized without clear evidence. Many mosquito species, Anopheles gambiae in particular, lay their eggs in breeding sites located around agricultural settings. There is a probability that, as a result of farming activities, insecticide residues may be found in soil and water, where they exercise a selection pressure on the larval stage of various populations of mosquitoes. To confirm this hypothesis, a study was conducted in the Republic of Benin to assess the environmental hazards which can be generated from massive use of pesticides in agricultural settings.Methods: Lacking an HPLC machine for direct quantification of insecticide residues in samples, this investigation was performed using indirect bioassays focussed on the study of factors inhibiting the normal growth of mosquito larvae in breeding sites. The speed of development was monitored as well as the yield of rearing An. gambiae larvae in breeding sites reconstituted with water and soil samples collected in agricultural areas known to be under pesticide pressure. Two strains of An. gambiae were used in this indirect bioassay: the pyrethroid-susceptible Kisumu strain and the resistant Ladji strain. The key approach in this methodology is based on comparison of the growth of larvae in test and in control breeding sites, the test samples having been collected from two vegetable farms.Results: Results obtained clearly show the presence of inhibiting factors on test samples. A normal growth of larvae was observed in control samples. In breeding sites simulated by using a few grams of soil samples from the two vegetable farms under constant insecticide treatments ( test samples), a poor hatching rate of Anopheles eggs coupled with a retarded growth of larvae and a low yield of adult mosquitoes from hatched eggs, was noticed.Conclusion: Toxic factors inhibiting the hatching of anopheles eggs and the growth of larvae are probably pesticide residues from agricultural practices. Samples used during this indirect assay have been stored in the laboratory and will be analysed with HPLC techniques to confirm hypothesis of this study and to identify the various end products found in soil and water samples from agricultural settings under pesticide pressure.