Environmental filtering decreases with fish development for the assembly of gut microbiota
Environmental filtering decreases with fish development for the assembly of gut microbiota
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随着鱼类肠道菌群组装的发育，环境过滤会减少
DOI：
10.1111/1462-2920.13365
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发表时间：
2016
影响因子：
5.1
通讯作者：
Zhou Jizhong
中科院分区：
文献类型：
--
作者：
Yan Qingyun;Li Jinjin;Yu Yuhe;Wang Jianjun;He Zhili;Van Nostr;Joy D.;Kempher Megan L.;Wu Liyou;Wang Yaping;Liao Lanjie;Li Xinghao;Wu Shu;Ni Jiajia;Wang Chun;Zhou Jizhong
肠道微生物群通常占据与海洋岛屿相当的具有明确界限/边界的栖息地。因此，肠道可以被视为一个“岛屿”，在周围环境的“海洋”中聚集微生物群落。本研究旨在揭示控制鱼肠“岛”生态系统中微生物群的生态机制。通过16S rRNA基因扩增子的高通量测序，分析了宿主发育过程中的分类组成、系统发育多样性和群落更替。结果表明，3种淡水鱼肠道菌群Shannon多样性随寄主发育均显著降低，优势菌群在寄主发育过程中也发生显著变化。零模型和基于系统发育的平均最近分类群距离（MNTD）分析表明，宿主肠道环境过滤导致了鱼类肠道“岛”中微生物群落的聚集。然而，随着鱼类的发展，当地群落的系统发育聚类和支配群落更替的确定性过程变得不那么明显。观察到的形成鱼类肠道微生物群的机制似乎主要是由肠道环境和伴随宿主发育过程的一些其他选择性变化形成的。这些发现极大地增强了我们对鱼类肠道生态系统中特定阶段的群落聚集模式的理解。
Gut microbiota typically occupy habitats with definable limits/borders that are comparable to oceanic islands. The gut therefore can be regarded as an ‘island’ for the assembly of microbial communities within the ‘sea’ of surrounding environments. This study aims to reveal the ecological mechanisms that govern microbiota in the fish gut ‘island’ ecosystem. Taxonomic compositions, phylogenetic diversity, and community turnover across host development were analyzed via the high‐throughput sequencing of 16S rRNA gene amplicons. The results indicate that the Shannon diversity of gut microbiota in the three examined freshwater fish species all significantly decreased with host development, and the dominant bacterial taxa also changed significantly during host development. Null model and phylogenetic‐based mean nearest taxon distance (MNTD) analyses suggest that host gut environmental filtering led to the assembly of microbial communities in the fish gut ‘island’. However, the phylogenetic clustering of local communities and deterministic processes that governed community turnover became less distinct as the fish developed. The observed mechanisms that shaped fish gut microbiota seemed to be mainly shaped by the gut environment and by some other selective changes accompanying the host development process. These findings greatly enhance our understanding of stage‐specific community assembly patterns in the fish gut ecosystem.