Chlorogenic acid, an antioxidant principle from the aerial parts ofArtemisia iwayomogi that acts on 1,1-diphenyl-2-picrylhydrazyl radical
Chlorogenic acid, an antioxidant principle from the aerial parts ofArtemisia iwayomogi that acts on 1,1-diphenyl-2-picrylhydrazyl radical
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DOI：
10.1007/bf02974002
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发表时间：
1997-04
影响因子：
6.7
通讯作者：
Soon Shin Kim;Chung-Kyu Lee;S. Kang;H. Jung;J. Choi
中科院分区：
文献类型：
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作者：
Soon Shin Kim;Chung-Kyu Lee;S. Kang;H. Jung;J. Choi
采用1，1-二苯基-2-苦基肼基（DPPH）自由基自由基自由基清除实验，研究了艾蒿甲醇提取物的抗氧化活性。Iwayomogi显示出强的抗氧化活性，因此用几种溶剂分级。各组分的抗氧化活性大小顺序为乙酸乙酯>正丁醇>水>氯仿>正己烷。抗氧化活性较强的乙酸乙酯和正丁醇可溶性组分经反复硅胶和Sephadex LH-20柱层析进一步纯化。从正丁醇部位中分离得到抗氧化活性成分之一绿原酸，以及非活性成分1-二十八烷醇、东莨菪碱、东莨菪碱、芹菜素7，4 ′-二-O-甲基醚、木犀草素6，3 ′-二-O-甲基醚、芹菜素7-甲基醚、2，4-二羟基-6-甲氧基苯乙酮4-O-β-D-葡萄糖苷和白坚木醇。绿原酸的抗氧化活性与L-抗坏血酸相当，L-抗坏血酸是一种众所周知的抗氧化剂。
The antioxidant activity ofArtemisia iwayomogiwas determined by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical The methanol extract ofA. iwayomogishowed strong antioxidant activity, and thus fractionated with several solvents. The antioxidant activity potential of the individual fraction was in the order of ethyl acetate>n-butanol>water>chloroform>n-hexane fraction. The ethyl acetate andn-butanol soluble fractions exhibiting strong antioxidant activity were further purified by repeated silica gel and Sephadex LH-20 column chromatography. Antioxidant chlorogenic acid was isolated as one of the active principles from then-butanol fraction, together with the inactive components, 1-octacosanol, scopoletin, scopolin, apigenin 7,4′-di-O-methylether luteolin 6,3′-di-O-methylether (jaceosidin), apigenin 7-methylether (genkwanin), 2,4-dihydroxy-6-methoxyacetophenone 4-O-β-D-glucopyranoside and quebrachitol. The antioxidant activity of chlorogenic acid was comparable to that of L-ascorbic acid, which is a well known antioxidant.