A Design Full of Holes: Functional Nanofilm-Coated Microdomains in Alginate Hydrogels.
A Design Full of Holes: Functional Nanofilm-Coated Microdomains in Alginate Hydrogels.
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DOI：
10.1039/c3tb20477d
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
McShane MJ
中科院分区：
文献类型：
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作者：
Roberts JR;Ritter DW;McShane MJ
这项研究证明了在聚电解质多层涂层微孔内含有多种胶囊的藻酸盐水凝胶的成功制造和功能表征。这些微孔海藻酸盐 (MPA) 水凝胶是通过使用聚电解质多层涂层 CaCO3 微球和弱酸性葡萄糖酸-δ-内酯对海藻酸盐进行一步内部离子凝胶化而制备的。在这里，通过共焦显微镜证实了模型大分子和荧光纳米粒子成功封装在微胶囊内（分布在较大的藻酸盐水凝胶中），同时通过扫描电子显微镜检查了 MPA 水凝胶的多孔形态。用未涂层的 CaCO3 微球构建的水凝胶将其内容物释放到周围环境中，而用聚电解质多层涂层的 CaCO3 微球构建的水凝胶将材料保留在孔内。含有模型酶葡萄糖氧化酶的 MPA 水凝胶保留了活性，并且能够与外部环境中的小分子发生反应。将各种功能材料封装在可模压、生物相容性藻酸盐基质中的能力使该方法在各种生物医学应用中具有极大的灵活性和潜力。
This study demonstrates the successful manufacture and functional characterization of alginate hydrogels containing a variety of encapsulates within polyelectrolyte multilayer-coated micropores. These microporous alginate (MPA) hydrogels are prepared via one-step internal ionotropic gelation of the alginate using polyelectrolyte multilayer-coated CaCO3 microspheres along with the weak acid glucono-δ-lactone. Here, successful encapsulation of a model macromolecule and fluorescent nanoparticles within microcapsules—distributed throughout the larger alginate hydrogel—is confirmed with confocal microscopy, while the porous morphology of the MPA hydrogels is examined with scanning electron microscopy. Hydrogels constructed with uncoated CaCO3 microspheres release their contents into the surrounding environment, while those constructed with polyelectrolyte multilayer-coated CaCO3 microspheres retain the materials within the pores. MPA hydrogels containing the model enzyme glucose oxidase retained activity and are capable of reacting with small molecules from the external environment. The ability to encapsulate an assortment of functional materials within a moldable, biocompatible alginate matrix gives this approach great flexibility and potential in a wide variety of biomedical applications.