Differentiation status dependent function of FOG‐1
Differentiation status dependent function of FOG‐1
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DOI：
10.1111/j.1365-2443.2004.00796.x
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发表时间：
2004-12
期刊：
影响因子：
2.1
通讯作者：
Makoto Tanaka;Jie Zheng;K. Kitajima;K. Kita;H. Yoshikawa;T. Nakano
中科院分区：
文献类型：
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作者：
Makoto Tanaka;Jie Zheng;K. Kitajima;K. Kita;H. Yoshikawa;T. Nakano
转录因子和辅助因子之间的分子相互作用在包括造血在内的各种生物过程中发挥着至关重要的作用。 FOG-1是GATA-1的同源辅因子，FOG-1/GATA-1复合物对于红系细胞和巨核细胞的造血分化至关重要。为了阐明 FOG-1 在不同细胞分化背景下的生物学功能，我们结合小鼠胚胎干 (ES) 细胞的体外分化和条件基因表达，分析了 FOG-1 表达对造血细胞分化的影响。 FOG-1 抑制所有分化阶段的原始红系细胞和定型红系细胞的增殖。然而，FOG-1通过不同的分子机制分别在早期和晚期分化阶段抑制和增强巨核细胞生成。此外，FOG-1抑制ES细胞的增殖，其分子机制与红细胞和巨核细胞不同。这些结果表明 FOG-1 以细胞分化背景依赖性方式发挥作用。
The molecular interactions between transcription factors and cofactors play crucial roles in various biological processes, including haematopoiesis. FOG‐1 is a cognate cofactor of GATA‐1, and the FOG‐1/GATA‐1 complex is essential for the haematopoietic differentiation of erythroid cells and megakaryocytes. In order to elucidate the biological functions of FOG‐1 in the different contexts of cell differentiation, we analysed the effects of FOG‐1 expression on haematopoietic cell differentiation, using a combination of in vitro differentiation of mouse embryonic stem (ES) cells and conditional gene expression. FOG‐1 suppressed the proliferation of primitive and definitive erythroid cells in all stages of differentiation. However, FOG‐1 inhibited and enhanced megakaryopoiesis in the early and late differentiation stages, respectively, through different molecular mechanisms. In addition, FOG‐1 inhibited the proliferation of ES cells, the molecular mechanism of which differs from those of erythroid and megakaryocytic cells. These results suggest that FOG‐1 functions in a cell differentiation context‐dependent manner.