Inhibition of growth and biofilm formation of clinical bacterial isolates by NiO nanoparticles synthesized from Eucalyptus globulus plants
Inhibition of growth and biofilm formation of clinical bacterial isolates by NiO nanoparticles synthesized from Eucalyptus globulus plants
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DOI：
10.1016/j.micpath.2017.09.019
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发表时间：
2017-10-01
影响因子：
3.8
通讯作者：
Musarrat, Javed
中科院分区：
文献类型：
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作者：
Saleem, Samia;Ahmed, Bilal;Musarrat, Javed
由于纳米颗粒 (NP) 独特的物理和化学特性，基于纳米技术的疗法已成为增强现有抗菌药物活性的一种有前途的方法。氧化镍纳米颗粒（NiO-NP）已被建议作为潜在的抗菌和抗肿瘤剂。在这项研究中，使用蓝桉叶提取物通过绿色方法合成了 NiO-NP，并评估了其杀菌活性。通过紫外-可见光、FT-IR、XRD、EDX 和电子显微镜等各种光谱技术测定的合成 NiO-NP 的形貌和纯度差异很大。合成的 NiO-NPs 是多形的，尺寸在 10 至 20 nm 之间变化。 XRD 分析显示 NiO-NPs 的平均尺寸为 19 nm。紫外-可见光谱数据显示 NiO-NP 具有很强的 SPR，特征光谱峰位于 396 nm。 FTIR 数据揭示了各种功能部分，如 C=C、C-N、C-H 和 O-H，阐明了叶子生物分子在 NiO-NP 封盖和分散中的作用。生物活性测定揭示了 NiO-NPs 对 ES beta L (+) 大肠杆菌、铜绿假单胞菌、甲氧西林敏感和耐药金黄色葡萄球菌的抗菌和抗生物膜活性。生长抑制测定表明，处理细胞的活力随时间和 NiO-NPs 浓度而降低。 NiO-NPs 诱导的生物膜抑制通过 PI 特征红色荧光的急剧增加来揭示，而 NiO-NPs 处理的细胞的 SEM 图像不规则收缩和扭曲，并有明显的凹陷/压痕。结果表明，NiO-NPs 具有显着的抗菌和抗生物膜活性，可能在影响人类健康的传染病的管理中发挥重要作用。 (C) 2017 Elsevier Ltd. 保留所有权利。
Nanotechnology based therapeutics has emerged as a promising approach for augmenting the activity of existing antimicrobials due to the unique physical and chemical properties of nanoparticles (NPs). Nickel oxide nanoparticles (NiO-NPs) have been suggested as prospective antibacterial and antitumor agent. In this study, NiO-NPs have been synthesized by a green approach using Eucalyptus globulus leaf extract and assessed for their bactericidal activity. The morphology and purity of synthesized NiO-NPs determined through various spectroscopic techniques like UV-Visible, FT-IR, XRD, EDX and electron microscopy differed considerably. The synthesized NiO-NPs were pleomorphic varying in size between 10 and 20 nm. The XRD analysis revealed the average size of NiO-NPs as 19 nm. The UV-Vis spectroscopic data showed a strong SPR of NiO-NPs with a characteristic spectral peak at 396 nm. The FTIR data revealed various functional moieties like C=C, C-N, C-H and O-H which elucidate the role of leaf biomolecules in capping and dispersal of NiO-NPs. The bioactivity assay revealed the antibacterial and anti-biofilm activity of NiO-NPs against ES beta L (+) E. coli, P. aeruginosa, methicillin sensitive and resistant S. aureus. Growth inhibition assay demonstrated time and NiO-NPs concentration dependent decrease in the viability of treated cells. NiO-NPs induced biofilm inhibition was revealed by a sharp increase in characteristic red fluorescence of PI, while SEM images of NiO-NPs treated cells were irregular shrink and distorted with obvious depressions/indentations. The results suggested significant antibacterial and antibiofilm activity of NiO-NPs which may play an important role in the management of infectious diseases affecting human health. (C) 2017 Elsevier Ltd. All rights reserved.