Distribution pattern of three neural calcium-binding proteins (NCS-1, VILIP and recoverin) in chicken, bovine and rat retina
Distribution pattern of three neural calcium-binding proteins (NCS-1, VILIP and recoverin) in chicken, bovine and rat retina
复制标题
三种神经钙结合蛋白（NCS-1、VILIP 和恢复蛋白）在鸡、牛和大鼠视网膜中的分布模式
DOI：
--
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
J. Cox
中科院分区：
文献类型：
--
作者：
S. Raad;M. Comte;P. Nef;S. Lenz;E. Gundelfinger;J. Cox
神经Ca ~（2+）结合蛋白（Neural Ca ~（2+）binding proteins，NCaPs）是4-EF-手蛋白的一个亚家族，在组织学和结构上呈现二分性：A型NCaPs选择性地表达于视网膜和松果体，呈现两个典型的EF-手; B型NCaPs存在于全脑，呈现三个规则的EF-手。在这项研究中，产生了针对A型NCaP恢复素（26 kDa）和B型NCaP VILIP和NCS-1（22 kDa）的抗血清。由于NCaPs之间的序列同一性高，特异性的多克隆抗体通过双交叉免疫亲和层析纯化; ELISA和免疫印迹分析确定，所得抗体显示出对其他两个相关NCaPs的选择性比低于1/363。此外，抗VILIP抗体对神经钙蛋白δ显示出一定的亲和力，抗恢复素抗体识别24 kDa的蛋白质，其最有可能是visinin。因此，鸡，大鼠和牛视网膜的免疫组织化学研究表明，抗recoverin抗体识别脊椎动物的光感受器和少数哺乳动物的双极细胞。抗VILIP抗体专门标记内视网膜，即无长突细胞和神经节细胞。NCS-1主要表达于感光细胞内节、内网状层和神经节细胞。NCS-1显示出最高的物种差异。NCS-1和VILIP的视网膜定位为了解其功能提供了重要的形态学基础。此外，针对NCaP的特异性抗体可以鉴定更复杂的神经组织（如大脑）中的细胞群。
SummaryNeural Ca2+-binding proteins (NCaPs) constitute a subfamily of 4-EF-hand proteins, and display a histological and structural dichotomy: the A-type NCaPs are selectively expressed by the retina and pineal organ and display two canonical EF-hands, whereas the B-type NCaPs are found in the entire brain and present three regular EF-hands. In this study, antisera were raised against the A-type NCaP recoverin (26 kDa) and the B-type NCaPs VILIP and NCS-1 (22 kDa). Since the sequence identity among NCaPs is high, specific polyclonal antibodies were purified by double cross-immunoaffinity chromatography; both ELISA and immunoblot analyses determined that the resulting antibodies showed selectivity ratios inferior to 1/363 for the two other related NCaPs. Besides, the anti-VILIP antibodies displayed some affinity toward neurocalcin δ, and the antirecoverin antibodies recognized a 24 kDa protein, which is most likely visinin. Thus, immunohistochemical studies on the chicken, rat and cow retina revealed that anti-recoverin antibodies recognized the vertebrate photoreceptors and a small number of mammalian bipolar cells. Anti-VILIP antibodies exclusively labelled the inner Retina, I.e. the amacrine and ganglion cells. NCS-1 was mainly present in the photoreceptor inner segments, the inner plexiform layer and the ganglion cells. NCS-1 showed the highest species disparity. The retinal localization of NCS-1 and VILIP offered an important morphological basis for the understanding of their function. Furthermore, specific antibodies against the NCaPs may enable the identification of cell populations in more complex neural tissues, such as the brain.
登录
查看更多内容
小鼠 23-kDa（一种视网膜钙结合蛋白（恢复蛋白））的发育外观、物种和组织特异性。
影响因子：
3.4
作者：
Stepanik,PL;Lerious,V;McGinnis,JF
通讯作者：
McGinnis,JF
恢复蛋白（而非维西宁）是人类视网膜中的一种自身抗原，与癌症相关的视网膜病变有关。
影响因子：
4.4
作者：
Polans,AS;Burton,MD;Haley,TL;Crabb,JW;Palczewski,K
通讯作者：
Palczewski,K
视觉钙传感器恢复蛋白的克隆、表达和结晶。
DOI：
10.1073/pnas.89.13.5705
发表时间：
1992
影响因子：
11.1
作者：
Ray,S;Zozulya,S;Niemi,GA;Flaherty,KM;Brolley,D;Dizhoor,AM;McKay,DB;Hurley,J;Stryer,L
通讯作者：
Stryer,L
DOI：
10.3233/clo-2008-0424
发表时间：
2008
期刊：
Cellular oncology : the official journal of the International Society for Cellular Oncology
影响因子：
--
作者：
D'Alessandro V;Muscarella LA;Copetti M;Zelante L;Carella M;Vendemiale G
通讯作者：
Vendemiale G