Structure of a cDNA for an acid phosphatase from phosphate-deficient lupin (Lupinus albus L.) Roots
Structure of a cDNA for an acid phosphatase from phosphate-deficient lupin (Lupinus albus L.) Roots
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磷酸盐缺乏的羽扇豆 (Lupinus albus L.) 根中酸性磷酸酶 cDNA 的结构
DOI：
10.1080/00380768.1999.10409358
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发表时间：
1999
影响因子：
2
通讯作者：
T. Tadano
中科院分区：
文献类型：
--
作者：
J. Wasaki;Masanori Omura;M. Osaki;Hiroyuki Ito;H. Matsui;T. Shinano;T. Tadano
从缺磷羽扇豆(Lupinus albus L.cv.)基因文库中克隆了一个酸性磷酸酶(EC 3.1.3.2；APase)的cDNA克隆，命名为LASAP1。基耶夫斯基)的根。LASAP1全长2,187个碱基，包括一个1,914个核苷酸的开放阅读框，编码638个氨基酸残基，包含一个31个氨基酸的信号序列。LASAP1编码的多肽是高度亲水的，而推测的信号肽是高度疏水的。预测该多肽与质膜具有较高的亲和力。推导的氨基酸序列与四季豆种子萌发的紫色APase和拟南芥分泌的紫色APase有很高的同源性。组成普通菜豆紫色APase活性中心的7个氨基酸在所有同源APase中都是保守的。Northern印迹分析表明，在缺磷条件下，相应的mRNA在地上部和根部均有积累。
Abstract We have isolated a cDNA clone, named LASAP1, for an acid phosphatase (EC 3.1.3.2; APase) from a cDNA library constructed from mRNA in phosphatedeficient lupin (Lupinus albus L. cv. Kievskij) roots. LASAP1 was 2,187 bp in length including a single open reading frame of 1,914 nucleotides that encodes 638 amino acid residues containing a putative signal sequence of 31 amino acids. The polypeptide encoded by LASAP1 was highly hydrophilic, while the putative signal peptide was highly hydrophobic. It was predicted that the peptide had a high affinity to the plasma membrane. The deduced amino acid sequence shared a high homology with that of purple APases from germinated seeds of Phaseolus vulgaris and those secreted from Arabidopsis thaliana. Seven amino acids composing the active center of the Phaseolus vulgaris purple APase were conserved in all the homologous APases. Northern blot analysis revealed that the corresponding mRNA accumulated in both shoots and roots under phosphate deficient conditions,...