In vivo on-line HPLC-microdialysis: simultaneous detection of monoamines and their metabolites in awake freely-moving rats
In vivo on-line HPLC-microdialysis: simultaneous detection of monoamines and their metabolites in awake freely-moving rats
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DOI：
10.1016/s0731-7085(98)00182-4
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发表时间：
1999-03-01
影响因子：
3.4
通讯作者：
Ashby, CR
中科院分区：
文献类型：
--
作者：
Chaurasia, CS;Chen, CE;Ashby, CR
建立了一种在线微孔高效液相色谱-电化学（HPLC-EC）检测法，用于多巴胺的同时测定（3，4-二羟基苯乙胺，DA）及其代谢产物3，4-二羟基苯乙酸（DOPAC）和高香草酸（3-甲氧基-4-羟基苯乙酸，HVA），以及血清素（5-羟色胺，5-HT）及其代谢产物5-羟吲哚-3-乙酸（5-HIAA）在清醒和自由活动大鼠纹状体内的分布。我们的方法能够检测DOPAC，5-HIAA，HVA，DA和5-HT在保留时间分别为3，5，6.5，9和24分钟，在重复的在线微透析采样。使用150 × 1 mm 5 μ m Cls微孔柱进行分析，该柱直接连接到包括6 mm玻璃碳工作电极的薄层径向流电化学电池（UniJet）上。为了适应信号输出（由于所研究的神经化学物质的变化水平），电流检测器配备有灵敏度可编程控制软件，用于在每个收集循环中无意地和重复地自动切换灵敏度。(C)1999 Elsevier Science B. V.保留所有权利。
An on-line microbore high performance liquid chromatographic-electrochemical (HPLC-EC) detection method has been developed for the simultaneous measurement of dopamine (3,4-dihydroxyphenethylamine, DA), its metabolites 3,4-dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (3-methoxy-4-hydroxyphenylacetic acid, HVA), as well as serotonin (5-hydroxytryptamine, 5-HT) and its metabolise 5-hydroxyindole-3-acetic acid (5-HIAA) in the striatum of awake and freely moving rats. Our method was capable of detecting DOPAC, 5-HIAA, HVA, DA and 5-HT at retention time of 3, 5, 6.5, 9, and 24 min, respectively, in repeated on-line microdialysis sampling. Analysis was performed using a 150 x 1 mm 5 mu m Cls microbore column attached directly to a thin-layer radial flow electrochemical cell (UniJet(R)) comprising a 6 mm glassy carbon working electrode. In order to accommodate signal outputs (due to a varying level in the neurochemicals under investigation), an amperometric detector was equipped with a sensitivity programmable controlling software for automatically switching sensitivity inattentively and repeatedly for each collection cycle. (C) 1999 Elsevier Science B.V. All rights reserved.