AMP-activated protein kinase regulates the expression of monocarboxylate transporter 4 in skeletal muscle
AMP-activated protein kinase regulates the expression of monocarboxylate transporter 4 in skeletal muscle
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DOI：
10.1016/j.lfs.2010.11.003
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发表时间：
2011-01-17
期刊：
影响因子：
6.1
通讯作者：
Iseki, Ken
中科院分区：
文献类型：
--
作者：
Furugen, Ayako;Kobayashi, Masaki;Iseki, Ken
目的：探讨一磷酸腺苷活化蛋白激酶激动剂5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside对大鼠骨骼肌和胚胎横纹肌肉瘤细胞株(RD细胞)单羧酸转运蛋白4(MCT4)表达的影响。通过Western blotting检测GLUT4和MCT4蛋白水平的变化。AICAR以纤维类型特异性的方式增加MCT4的mRNA和蛋白水平。在一项体外研究中，AICAR提高了MCT4的mRNA和蛋白水平。此外，AICAR诱导的MCT4表达可被AMPK抑制剂化合物C阻断。意义：在本研究中，我们发现AMPK激活以纤维类型特异性的方式诱导RD细胞和大鼠骨骼肌MCT4的表达。这些结果表明AMPK介导的途径可能参与了骨骼肌中MCT4的表达。(C)2010 Elsevier Inc.保留所有权利。
Aims: The aim of this study was to determine the effect of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR), an AMP-activated protein kinase (AMPK) activator, on monocarboxylate transporter 4 (MCT4) expression in rat skeletal muscle and a prototypic embryonal rhabdomyosarcoma cell line (RD cells).Main methods: We examined the alteration in Glucose transporter 4 (GLUT4) and MCT4 mRNA levels by quantitative real-time PCR. Alteration in GLUT4 and MCT4 protein levels was examined by Western blotting.Key findings: In an in vivo study. AICAR increased MCT4 mRNA and protein levels in a fiber-type specific manner. In an in vitro study, AICAR increased MCT4 mRNA and protein levels. Moreover, AICAR-induced MCT4 expression was blocked by Compound C, an AMPK inhibitor.Significance: In this study, we found that AMPK activation induced expression of MCT4 in RD cells and rat skeletal muscle in a fiber-type specific manner. These results indicate the possible involvement of an AMPK-mediated pathway associated with MCT4 expression in skeletal muscle. (C) 2010 Elsevier Inc. All rights reserved.