In vivo determination of vitamin d function using transgenic mice carrying a human osteocalcin luciferase reporter gene.
In vivo determination of vitamin d function using transgenic mice carrying a human osteocalcin luciferase reporter gene.
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DOI：
10.1155/2013/895706
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发表时间：
2013
影响因子：
--
通讯作者：
Sato K
中科院分区：
文献类型：
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作者：
Nakanishi T;Saito R;Taniguchi M;Oda H;Soma A;Yasunaga M;Yamane M;Sato K
维生素D是骨化的重要因素，缺乏维生素D会导致佝偻病。骨钙素是骨基质中发现的一种非胶原蛋白，参与矿化和钙离子稳态，是主要的骨形态发生标志物之一，用于评估成骨细胞成熟和成骨激活。我们建立了在 10-kb 骨钙素增强子/启动子序列控制下表达荧光素酶的转基因小鼠系。使用这些转基因小鼠，我们通过体内生物发光评估了活性形式的维生素 D2 和 D3 的骨形态发生功能。结果，观察到1α,25-羟基维生素D3具有很强的骨化活性。我们的小鼠系统可以提供一种可行的检测方法，通过无创筛选评估功能性食品和药物开发中的成骨活性。
Vitamin D is an essential factor for ossification, and its deficiency causes rickets. Osteocalcin, which is a noncollagenous protein found in bone matrix and involved in mineralization and calcium ion homeostasis, is one of the major bone morphogenetic markers and is used in the evaluation of osteoblast maturation and osteogenic activation. We established transgenic mouse line expressing luciferase under the control of a 10-kb osteocalcin enhancer/promoter sequence. Using these transgenic mice, we evaluated the active forms of vitamins D2 and D3 for their bone morphogenetic function by in vivo bioluminescence. As the result, strong activity for ossification was observed with 1α,25-hydroxyvitamin D3. Our mouse system can offer a feasible detection method for assessment of osteogenic activity in the development of functional foods and medicines by noninvasive screening.