The fate of cryopreserved adipose aspirates after in vivo transplantation.
The fate of cryopreserved adipose aspirates after in vivo transplantation.
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DOI：
10.1016/j.asj.2006.10.005
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发表时间：
2006-11-01
影响因子：
2.9
通讯作者：
Gao, Dayong
中科院分区：
文献类型：
--
作者：
Pu, Lee L Q;Cui, Xiangdong;Gao, Dayong
背景：脂肪组织的成功长期保存可能对自体脂肪移植未来的临床应用产生重要影响。本课题组最近开发了一种最佳的低温保存脂肪吸入物的方法，以可能长期保存脂肪吸入物。目的：本研究的目的是评估先前冷冻保存的脂肪吸入物在已建立的裸鼠模型中的命运。方法：脂肪吸入物取自患者经离心后的腹部整形脂肪成形术。新鲜对照组(n=20)，将新鲜脂肪抽吸物注入裸鼠头皮后部。在最佳冷冻保存组(n=20)，注射最佳冷冻保存后的脂肪抽吸物。单纯冻存组(n=20)，单纯冻存后注入脂肪抽提物。观察每组动物头皮后皮下脂肪移植物的大体形态，观察时间长达16周。结果：与单纯超低温保存组相比，最佳超低温保存组移植脂肪体积、重量和脂肪组织结构的维持情况明显好于单纯超低温保存组，但仍不如新鲜对照组。结论：在活体实验的基础上，我们认为本实验室建立的最佳超低温保存方法能够较好地长期保存脂肪吸入物。然而，进一步的研究可能仍有必要改进我们的方法，以优化脂肪组织的冷冻保存。
BACKGROUND: Successful long-term preservation of adipose tissues may have an important impact on future clinical application of autologous fat transplantation. Our group has recently developed an optimal cryopreservation method for possible long-term preservation of adipose aspirates.OBJECTIVE: The purpose of this study was to evaluate the fate of previously cryopreserved adipose aspirates after in vivo administration in an established nude mouse model.METHODS: Adipose aspirates were collected from a cosmetic lipoplasty of the patient's abdomen after centrifugation. In the fresh control group (n = 20), fresh adipose aspirates were injected into the posterior scalp of a nude mouse. In the optimal cryopreservation group (n = 20), adipose aspirates after the optimal cryopreservation were injected. In the simple cryopreservation group (n = 20), adipose aspirates after the simple cryopreservation were injected. All animals in each group were observed for gross appearance of maintained fat grafts over their posterior scalps for up to 16 weeks. The final volume and weight of maintained fat grafts and their histology were evaluated at the end of the study.RESULTS: More maintained volume, weight, and fatty tissue structure of injected free grafts were found in the optimal cryopreservation group compared with the simple cryopreservation group, but the results were still less satisfactory than those in the fresh control group.CONCLUSIONS: Based on this in vivo study, we believe that an optimal cryopreservation method developed in our laboratory provides reasonably good long-term preservation of adipose aspirates. However, further studies may still be warranted to refine our method for optimal cryopreservation of adipose tissues.