In vitro model for the study of necrosis and apoptosis in native cartilage
In vitro model for the study of necrosis and apoptosis in native cartilage
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DOI：
10.1002/path.1169
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发表时间：
2002-09-01
影响因子：
7.3
通讯作者：
Mainil-Varlet, P
中科院分区：
文献类型：
--
作者：
Grogan, SP;Aklin, B;Mainil-Varlet, P
细胞凋亡在从早期发育到衰老和许多疾病状态的所有方面都发挥着作用。在体内状态下研究这一重要过程对于了解其不同的作用并开辟进一步的治疗干预途径至关重要。本文提出了一种离体牛关节软骨模型，研究急性损伤后的凋亡和坏死过程。离体牛关节软骨评估后1，3和6天钬：YAG激光治疗（780 mJ）。单独或以各种组合使用用于可视化细胞活力、半胱天冬酶-3活性、线粒体膜电位变化和DNA片段化程度（TUNEL测定）的标记物。还进行了标准组织学和透射电子显微镜（TEM）检查，以进行更全面的评估。重大进展（P
Apoptosis plays a role in everything from early development to ageing and in a host of disease states. Studying this important process in the in vivo state is critical, to understand its varied role and to open further avenues of therapeutic intervention. The present paper presents an ex vivo bovine articular cartilage model to study apoptotic and necrotic processes following acute injury. Ex vivo bovine articular cartilage was assessed 1, 3 and 6 days following holmium: YAG laser treatment (780 mJ). Markers to visualize cell viability, caspase-3 activity, changes in mitochondrial membrane potential and the degree of DNA fragmentation (TUNEL assay) were used alone or in various combinations. Standard histology and transmission electron microscopy (TEM) were also performed for a more comprehensive assessment. A significant progression (p