Segregated water observed in a putative fish embryo cryopreservative.
Segregated water observed in a putative fish embryo cryopreservative.
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DOI：
10.1098/rsos.150655
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发表时间：
2016-03
影响因子：
3.5
通讯作者：
Holt WV
中科院分区：
文献类型：
--
作者：
Kirichek O;Soper AK;Dzyuba B;Holt WV
新的低温保存策略的发展在医学和农业方面具有巨大的潜力，对于目前无法保存的濒危物种的保护至关重要。任何潜在的冷冻保存溶液的关键性质是其在冷却和随后的加热期间防止细胞损伤冰形成的能力。这项研究的重点是有前途的模型防冻液的冻结行为。我们进行中子散射分析，结合计算机建模，淬火冷却这些解决方案后的水结构。发现该溶液中的水形成由冷冻保护剂溶质分子的周围基质包封的纳米簇。我们认为，这些小体积抑制冰的形成，因为水没有空间的结构弛豫所需的冷却过程的时间尺度上结晶。
Development of new cryopreservation strategies has major potential in medicine and agriculture and is critical to the conservation of endangered species that currently cannot be preserved. A critical property of any potential cryopreservative solution is its ability to prevent cell-damaging ice formation during cooling and subsequent heating. This study focuses on the freezing behaviour of promising model cryoprotective solutions. We perform neutron scattering analysis, combined with computer modelling, of the water structure after quench cooling these solutions. It is found that water in this solution forms nano-clusters encapsulated by the surrounding matrix of cryoprotectant solute molecules. We posit that these small volumes inhibit ice formation, because water does not have space for the structural relaxation required to crystallize on the timescale of the cooling process.