Polyvalent choline phosphate as a universal biomembrane adhesive.
Polyvalent choline phosphate as a universal biomembrane adhesive.
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DOI：
10.1038/nmat3272
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发表时间：
2012-05
期刊：
影响因子：
41.2
通讯作者：
Xifei Yu;Zong-hua Liu;J. Janzen;Irina Chafeeva;Sonja A Horte;Wei Chen;R. Kainthan;J. Kizhakkedathu-J.-Kizhak
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文献类型：
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作者：
Xifei Yu;Zong-hua Liu;J. Janzen;Irina Chafeeva;Sonja A Horte;Wei Chen;R. Kainthan;J. Kizhakkedathu-J.-Kizhak
所有真核细胞的细胞膜中的磷脂含有磷脂酰胆碱（PC）作为头基。在这里，我们表明，超支化聚甘油（HPGs）装饰的“PC-反”磷酸胆碱（CP）在多价的方式可以静电结合到各种细胞膜和PC-含有脂质体，结合强度取决于每个大分子的CP基团的数量密度。我们还表明，HPG-CP可以导致细胞粘附与其他细胞的不同亲和力，并结合可以通过随后暴露于携带少量PC的低分子量HPGs逆转。此外，富含PC的膜吸附并迅速内化荧光HPG-CP，但不是HPG-PC分子，这表明HPG-CP可用作药物递送剂。CP修饰的聚合物应该有广泛的用途，例如作为组织密封剂和脂质纳米结构的自组装。
Phospholipids in the cell membranes of all eukaryotic cells contain phosphatidyl choline (PC) as the headgroup. Here we show that hyperbranched polyglycerols (HPGs) decorated with the ’PC-inverse’ choline phosphate (CP) in a polyvalent fashion can electrostatically bind to a variety of cell membranes and to PC-containing liposomes, the binding strength depending on the number density of CP groups per macromolecule. We also show that HPG–CPs can cause cells to adhere with varying affinity to other cells, and that binding can be reversed by subsequent exposure to low molecular weight HPGs carrying small numbers of PCs. Moreover, PC-rich membranes adsorb and rapidly internalize fluorescent HPG–CP but not HPG–PC molecules, which suggests that HPG–CPs could be used as drug-delivery agents. CP-decorated polymers should find broad use, for instance as tissue sealants and in the self-assembly of lipid nanostructures.