BACTERIOPHAGE LAMBDA-MEDIATED TRANSPOSON MUTAGENESIS OF PHYTOPATHOGENIC AND EPIPHYTIC ERWINIA SPECIES IS STRAIN DEPENDENT
BACTERIOPHAGE LAMBDA-MEDIATED TRANSPOSON MUTAGENESIS OF PHYTOPATHOGENIC AND EPIPHYTIC ERWINIA SPECIES IS STRAIN DEPENDENT
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DOI：
10.1007/bf00332415
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发表时间：
1989-09-01
期刊：
影响因子：
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通讯作者：
SALMOND, GPC
中科院分区：
文献类型：
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作者：
ELLARD, FM;CABELLO, A;SALMOND, GPC
利用转化和接合动员的方法，将携带大肠杆菌lamb基因的质粒转移到一系列欧文氏菌中。将产生的菌株感染波长467，并筛选卡那霉素抗性转导子的各种突变表型，包括营养不良和胞外酶活性改变。逆转分析表明，大多数突变表型是由于Tn5插入所致。这些技术的适用性高度依赖于应变。然而，获得了一种快速而简单的突变分离方法，这可以允许在几个重要物种中使用其他与波长相关的基因技术，这些物种到目前为止还没有被基因操纵。
Using transformation and conjugal mobilization, plasmids carrying the lamB gene of Escherichia coli were transferred to a range of Erwinia strains. The resultant strains were infected with .lambda.467, and kanamycin resistant transductants were screened for various mutant phenotypes including auxotrophy and altered extracellular enzyme activities. Reversion analysis suggested that most mutant phenotypes were due to Tn5 insertion. The applicability of the techniques was highly strain dependent. However a rapid and simple route to mutant isolation was obtained, which could allow the use of other .lambda.-related genetic techniques in several important species which, to date, have not been genetically manipulated.