Induction of the perceptual correlate of human long-term potentiation (LTP) is associated with the 5-HTT genotype
Induction of the perceptual correlate of human long-term potentiation (LTP) is associated with the 5-HTT genotype
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DOI：
10.1016/j.brainres.2012.10.045
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发表时间：
2013-01-23
期刊：
影响因子：
2.9
通讯作者：
Gjerstad, Johannes
中科院分区：
文献类型：
--
作者：
Matre, Dagfinn;Olsen, Maria Belland;Gjerstad, Johannes
本研究的目的是研究如何在SLC 6A 4基因的启动子编码5-羟色胺转运体（5-HTT）的遗传变异可能会影响诱导的长时程增强（LTP）。采用TaqMan法和凝胶电泳法对53名健康志愿者进行基因分型。根据转录率，将受试者分为3组：5-HTT SS、5-HTT SLG/LALG/SLA和5-HTT LALA。在视觉模拟量表（VAS）上评定测试刺激的疼痛强度。高频刺激（HFS）条件反射应用于一个手臂被用来诱导LTP。通过调节电极传递的电测试刺激诱发HFS调节后，仅观察到疼痛的微小变化。此外，通过条件电极传递的测试刺激引起的疼痛的变化与5-HTT基因型无关。然而，我们观察到一个明确的增加疼痛后，HFS条件诱发的机械针刺测试刺激附近的皮肤提供的条件。此外，9个人与5-HTT SS基因型比5-HTT SLG/LALG/SLA基因型的个人HFS条件机械针刺试验刺激后，报告更多的疼痛。因此，目前的数据表明，人类LTP的感知相关的诱导与编码5-HTT的基因的遗传变异性有关。综上所述，这表明5-HTT的表达对于人类LTP的诱导可能是重要的。(C)2012爱思唯尔有限公司版权所有。
The purpose of the present study was to examine how genetic variability in the promoter of the SLC6A4 gene encoding the serotonin transporter (5-HTT) may influence induction of long-term potentiation (LTP). The genotyping of the 53 healthy volunteers was performed by a combination of TaqMan assay and gel electrophoresis. Based on the transcription rates, the subjects were divided in 3 groups; 5-HTT SS, 5-HTT SLG/LALG/SLA and 5-HTT LALA. The intensity of pain to test stimuli was rated on a visual analog scale (VAS). High frequency stimulation (HFS) conditioning applied to one arm was used to induce LTP. Only a minor change in pain was observed following the HFS conditioning evoked by electrical test stimuli delivered through the conditioning electrode. Moreover, the change in pain evoked by test stimuli delivered through the conditioning electrode was not related to the 5-HTT genotype. However, we observed a clear increase in pain following the HFS conditioning evoked by mechanical pin-prick test stimuli delivered at the skin adjacent to the conditioning. Also, the 9 individuals with the 5-HTT SS genotype reported more pain than individuals with 5-HTT SLG/LALG/SLA genotype following HFS conditioning on mechanical pin-prick test stimuli. Thus, the present data show that induction of the perceptual correlate of human LTP is associated with the genetic variability in the gene encoding the 5-HTT. Taken together, this suggests that the expression of 5-HTT, may be important for induction of LTP in humans. (C) 2012 Elsevier B.V. All rights reserved.