Analysis of microsatellite polymorphism in inbred knockout mice.
Analysis of microsatellite polymorphism in inbred knockout mice.
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近交基因敲除小鼠微卫星多态性分析
DOI：
10.1371/journal.pone.0034555
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Chen Z
中科院分区：
文献类型：
--
作者：
Zuo B;Du X;Zhao J;Yang H;Wang C;Wu Y;Lu J;Wang Y;Chen Z
以前，我们发现，42的基因型的198个小鼠微卫星位点，分布在除Y染色体以外的所有染色体，改变从单态到多态性（CMP）的转基因近交系小鼠品系。在本研究中，我们进一步研究了CMP是否也与基因敲除（KO）小鼠品系的同源重组有关。采用聚合酶链反应（PCR）技术，通过短串联重复序列（STR）扫描和直接序列克隆技术，对29个KO近交系小鼠的42个微卫星位点进行了分析，以证明微卫星多态性。选择这29只KO小鼠来源的C57 BL/6 J和129小鼠品系作为背景对照。结果表明，42个位点中有10个位点（23.8%）在部分品系小鼠中出现CMP。除D3 Mit 22的三核苷酸重复位点在9号品系中具有微卫星CMP外，其余41个位点的核心序列均为二核苷酸重复，其中9个位点（21.95%）具有微卫星CMP。然而，29只KO小鼠品系中有11只（37.9%）被认为具有CMP。CMP中常见的二核苷酸基序是（TG）n（50%，2/4），其次是（GT）n（27.27%，3/11）和（CA）n（23.08%，3/13）。（CT）n和（AG）n重复的微卫星CMP均为20%（1/5）。根据克隆测序结果，6个KO小鼠品系显示核苷酸插入，1个显示核苷酸缺失。此外，2个位点（D13 Mit 3和D14 Mit 102）在2个品系中显示CMP，9号小鼠同时在2个位点（D3 Mit 22和D13 Mit 3）显示CMP。总的来说，这些结果表明，微卫星多态性存在于检查的近交KO小鼠。
Previously, we found that the genotype of 42 out of 198 mouse microsatellite loci, which are distributed among all chromosomes except the Y chromosome, changed from monomorphism to polymorphism (CMP) in a genetically modified inbred mouse strain. In this study, we further examined whether CMP also relates to the homologous recombination in gene knockout (KO) mouse strains. The same 42 microsatellite loci were analyzed by polymerase chain reaction (PCR) in 29 KO inbred mouse strains via short tandem sequence repeat (STR) scanning and direct sequence cloning to justify microsatellite polymorphisms. The C57BL/6J and 129 mouse strains, from which these 29 KO mice were derived, were chosen as the background controls. The results indicated that 10 out of 42 (23.8%) loci showed CMP in some of these mouse strains. Except for the trinucleotide repeat locus of D3Mit22, which had microsatellite CMP in strain number 9, the core sequences of the remaining 41 loci were dinucleotide repeats, and 9 out of 41 (21.95%) showed CMPs among detected mouse strains. However, 11 out of 29 (37.9%) KO mice strains were recognized as having CMPs. The popular dinucleotide motifs in CMP were (TG)n (50%, 2/4), followed by (GT)n (27.27%, 3/11) and (CA)n (23.08%, 3/13). The microsatellite CMP in (CT)n and (AG)n repeats were 20% (1/5). According to cloning sequencing results, 6 KO mouse strains showed insertions of nucleotides whereas 1 showed a deletion. Furthermore, 2 loci (D13Mit3 and D14Mit102) revealed CMP in 2 strains, and mouse strain number 9 showed CMPs in two loci (D3Mit22 and D13Mit3) simultaneously. Collectively, these results indicated that microsatellite polymorphisms were present in the examined inbred KO mice.
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DOI：
10.1073/pnas.91.19.8905
发表时间：
1994-09-13
影响因子：
11.1
作者：
AQUILINA, G;HESS, P;BIGNAMI, M
通讯作者：
BIGNAMI, M
影响因子：
64.8
作者：
BRONNER, CE;BAKER, SM;LISKAY, RM
通讯作者：
LISKAY, RM
影响因子：
15.1
作者：
Eisener-Dorman, Amy F.;Lawrence, David A.;Bolivar, Valerie J.
通讯作者：
Bolivar, Valerie J.
影响因子：
2.6
作者：
Day, Tanya K.;Hooker, Antony M.;Sykes, Pamela J.
通讯作者：
Sykes, Pamela J.
影响因子：
64.8
作者：
TAUTZ, D;TRICK, M;DOVER, GA
通讯作者：
DOVER, GA