Utility of Amorphous Calcium Phosphate-Based Scaffolds in Dental/Biomedical Applications.
Utility of Amorphous Calcium Phosphate-Based Scaffolds in Dental/Biomedical Applications.
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发表时间：
2017
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通讯作者：
D. Bienek;D. Škrtić
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作者：
D. Bienek;D. Škrtić
磷酸钙(CaP)材料是生物硬组织中重要的无机成分。包括无定形磷酸钙(ACP)在内的CAPS已广泛应用于牙科和生物医学领域，如组织工程。支架结构通常用作模板来创建仿生环境。本文综述了ACP支架的制备技术，包括固有掺入ACP的组织工程构建技术，以及通过在基质上沉淀矿化溶液形成的支架。重点介绍了用于评估ACP支架引起的细胞和分子反应的方法，如生物相容性、细胞传导性、细胞粘附性、细胞分化、表型特征和基因表达。复合ACP支架的生物活性可以通过加入生物分子来创建多功能性能来增强。在此，我们综述了抗生素、生长因子和基因传递系统在构建多功能ACP支架方面的应用。由于CAP材料作为药物输送系统已经被研究了很多年，我们简要地考虑了将这些系统与现有的ACP支架结构集成的潜力以及用于精确医学的潜力。
Calcium phosphate (CaP) materials are important inorganic constituents in biological hard tissue. CaPs, including amorphous calcium phosphate (ACP) have been widely applied in dental and biomedical applications, such as tissue engineering. Scaffold constructs are commonly used as templates to create a biomimetic environment. This review considers ACP scaffold fabrication techniques, including tissue-engineered constructs with intrinsic incorporation of ACP as well as scaffolds formed via precipitation of mineralized solutions on a substrate. Attention is given to the approaches used to assess cellular and molecular responses elicited by ACP scaffolds, such as biocompatibility, cell conductivity, cell adhesion, cell differentiation, phenotypic profiles, and gene expression. Bioactivity of composite ACP scaffolds can be enhanced by incorporating biomolecules to create multi-functional properties. Herein we summarize the use of antibiotics, growth factors, and gene delivery systems to create multi-functional ACP scaffolds. Inasmuch as CaP materials have been investigated as drug delivery systems for many years, we briefly consider the potential of integrating these systems with existing ACP scaffold constructs and the potential for precision medicine.