Local Translation in Perisynaptic Astrocytic Processes Is Specific and Changes after Fear Conditioning
Local Translation in Perisynaptic Astrocytic Processes Is Specific and Changes after Fear Conditioning
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DOI：
10.1016/j.celrep.2020.108076
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发表时间：
2020-08-25
期刊：
影响因子：
8.8
通讯作者：
Cohen-Salmon, Martine
中科院分区：
文献类型：
--
作者：
Mazare, Noemie;Oudart, Marc;Cohen-Salmon, Martine
局部翻译是一种保守的机制，赋予细胞快速响应局部刺激的能力。在大脑中，最近有报道称它存在于星形胶质细胞中，星形胶质细胞的精细过程与血管和突触接触。然而，星形胶质细胞局部翻译的特异性和调节仍然未知。我们研究海马突触周围星形胶质细胞过程（PAP），并表明它们包含翻译的机器。使用一个完善的免疫沉淀技术，我们的特点是整个池的核糖体结合的mRNA在PAP和比较它在整个星形胶质细胞中表达。我们发现，一个特定的池的mRNA是高度极化的突触接口。这些转录本编码一个意想不到的分子库，由参与铁稳态，翻译，细胞周期和细胞骨架的蛋白质组成。值得注意的是，我们观察到恐惧条件反射后一些PAP富集转录物的全球RNA分布和核糖体结合状态的改变，表明星形胶质细胞局部翻译在记忆和学习中的作用。
Local translation is a conserved mechanism conferring cells the ability to quickly respond to local stimuli. In the brain, it has been recently reported in astrocytes, whose fine processes contact blood vessels and synapses. Yet the specificity and regulation of astrocyte local translation remain unknown. We study hippocampal perisynaptic astrocytic processes (PAPs) and show that they contain the machinery for translation. Using a refined immunoprecipitation technique, we characterize the entire pool of ribosome-bound mRNAs in PAPs and compare it with the one expressed in the whole astrocyte. We find that a specific pool of mRNAs is highly polarized at the synaptic interface. These transcripts encode an unexpected molecular repertoire, composed of proteins involved in iron homeostasis, translation, cell cycle, and cytoskeleton. Remarkably, we observe alterations in global RNA distribution and ribosome-bound status of some PAP-enriched transcripts after fear conditioning, indicating the role of astrocytic local translation in memory and learning.