Genetic studies of membrane excitability in Drosophila: lethal interaction between two temperature-sensitive paralytic mutations.
Genetic studies of membrane excitability in Drosophila: lethal interaction between two temperature-sensitive paralytic mutations.
复制标题
果蝇膜兴奋性的遗传学研究：两种温度敏感麻痹突变之间的致死相互作用。
DOI：
10.1093/genetics/108.4.897
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发表时间：
1984
期刊：
影响因子：
3.3
通讯作者：
Ganetzky,B
中科院分区：
文献类型：
--
作者：
Ganetzky,B
果蝇的两个突变体parats 1（1-53.9）和napts（2-56.2）都表现出与神经动作电位传导阻滞相关的相似的温度敏感性麻痹，表明这两个基因产物具有相似的功能。这一观点得到了双突变体无条件致命的观察结果的支持。对这种协同作用的遗传分析表明：1）它特异地涉及到para 1和naploci;（2）所有的para 1等位基因都与napts相互作用，但相互作用的强度以等位基因依赖的方式变化;（3）双突变体的致死性发生在parats 1的第一龄幼虫，但与其他para 1等位基因不同;（4）低剂量的para+在apts背景下引起致死性。这些结果与先前对这些突变体的电生理、行为和药理学研究一起表明，对和萘都影响钠通道，并且可能编码不同的亚基。
Two mutants ofDrosophila melanogaster, parats1(1-53.9) andnapts(2-56.2) both display similar temperature-sensitive paralysis associated with blockage in the conduction of nerve action potentials, suggesting that the two gene products have a similar function. This idea is supported by the observation that the double mutant is unconditionally lethal. Genetic analysis of this synergistic interaction has revealed the following: 1) it specifically involves theparaandnaploci; (2) allparaalleles interact withnapts, but the strength of the interaction varies in an allele-dependent fashion; (3) lethality of the double mutant occurs during the first larval instar withparats1but differs with otherparaalleles; (4) hypodosage ofpara+causes lethality in anaptsbackground. These results together with previous electrophysiological, behavioral and pharmacological studies of these mutants suggest that bothparaandnapaffect sodium channels and possibly encode different subunits.