EGCG regulates fatty acid metabolism of high-fat diet-fed mice in association with enrichment of gut Akkermansia muciniphila
EGCG regulates fatty acid metabolism of high-fat diet-fed mice in association with enrichment of gut Akkermansia muciniphila
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EGCG 调节高脂饮食喂养小鼠的脂肪酸代谢，与肠道阿克曼氏菌富集相关
DOI：
10.1016/j.jff.2020.104261
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发表时间：
2020-12-01
影响因子：
5.6
通讯作者：
Yang, Xingbin
中科院分区：
文献类型：
--
作者：
Liu, Xiaoxia;Zhao, Ke;Yang, Xingbin
本研究旨在探讨表没食子儿茶素没食子酸酯对脂肪酸代谢的影响及其与肠道菌群的可能关系。给喂食HFD的C57 BL/6 J小鼠施用210 mg/kg的EGCG，持续12周。EGCG显著降低血清中饱和脂肪酸（C17：0和C22：0），增加不饱和脂肪酸（C18：1和C18：2）（p < 0.05），并降低SCD 1和FADS 2的肝脏蛋白表达。施用EGCG显著地富集了疣微菌，并减少了厚壁菌门和Saccharomyces细菌（p < 0.05）。拟杆菌、嗜热单胞菌和阿克曼氏菌与血清脂肪酸谱呈强相关（p < 0.05，r > 0.45或r < -0.45）。有趣的是，嗜粘蛋白阿克曼氏菌与C18：2正相关（p < 0.05，r = 0.51），与C22：0负相关（p < 0.05，r =-0.48）。这些结果表明，改变循环中的长链脂肪酸谱参与EGCG介导的脂质代谢的HFD喂养的小鼠，肠道嗜粘蛋白阿克曼氏菌可能在这个过程中发挥重要作用。
This study is to examine the effects of EGCG on fatty acid metabolism and their possible relationship with gut microbiota. C57BL/6J mice fed with HFD were administrated with 210 mg/kg EGCG for 12 weeks. EGCG significantly decreased saturated fatty acids (C17:0 and C22:0), increased the unsaturated fatty acids (C18:1 and C18:2) (p < 0.05) in the serum, and decreased the liver protein expression of SCD1 and FADS2. EGCG administration significantly enriched the Verrucomicrobia and decreased the Firmicutes and Saccharibacteria (p < 0.05). Bacteroides, Candidatus_Saccharimonas and Akkermansia showed strong correlation with serum fatty acids profile (p < 0.05, r > 0.45 or r < -0.45). Interestingly, Akkermansia muciniphila was positively correlated with C18:2 (p < 0.05, r = 0.51) and negatively correlated with C22:0 (p < 0.05, r =-0.48). These findings demonstrate that alteration of the circulating long chain fatty acids profile is involved in EGCGmediated lipid metabolism of HFD-fed mice, and the intestinal Akkermansia muciniphila bacteria might play an important role in this process.