Pressure-driven release of viral genome into a host nucleus is a mechanism leading to herpes infection
Pressure-driven release of viral genome into a host nucleus is a mechanism leading to herpes infection
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DOI：
10.7554/elife.47212.001
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发表时间：
2019-08-08
期刊：
影响因子：
7.7
通讯作者：
Evilevitch, Alex
中科院分区：
文献类型：
--
作者：
Brandariz-Nunez, Alberto;Liu, Ting;Evilevitch, Alex
许多病毒先前已被证明在其病毒蛋白质外壳（称为衣壳）内具有加压基因组。这种压力是由于衣壳内带负电荷的病毒核酸的紧密限制造成的。然而，衣壳压力与病毒感染的相关性尚未得到证实。在这项工作中，我们表明疱疹病毒衣壳内数十个大气压的内部DNA压力有助于将病毒基因组喷射到宿主细胞核中。据我们所知，这首次证明了病毒基因组渗透到宿主细胞核中的压力依赖性机制，导致真核细胞感染。
Many viruses previously have been shown to have pressurized genomes inside their viral protein shell, termed the capsid. This pressure results from the tight confinement of negatively charged viral nucleic acids inside the capsid. However, the relevance of capsid pressure to viral infection has not been demonstrated. In this work, we show that the internal DNA pressure of tens of atmospheres inside a herpesvirus capsid powers ejection of the viral genome into a host cell nucleus. To our knowledge, this provides the first demonstration of a pressure-dependent mechanism of viral genome penetration into a host nucleus, leading to infection of eukaryotic cells.