Considerations for two inoculation methods of rabbit hepatic tumors: Pathology and image features
Considerations for two inoculation methods of rabbit hepatic tumors: Pathology and image features
复制标题
兔肝肿瘤两种接种方法的考虑：病理学和图像特征
DOI：
10.3892/etm.2011.435
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发表时间：
2012-03-01
影响因子：
2.7
通讯作者：
Zheng, Shu-Sen
中科院分区：
文献类型：
--
作者：
Sun, Jun-Hui;Zhang, Yue-Lin;Zheng, Shu-Sen
兔VX2肝肿瘤模型已广泛用于肝癌的研究。本研究旨在比较两种接种方法，以确定建立兔肝肿瘤的改进方式。总共44只兔子随机分为2组。所有接种均通过开腹手术进行。在第 1,22 组兔子中，接受了 0.2 ml 体积的 VX2 肿瘤细胞悬液，使用 18 号针将其缓慢注射到兔子肝实质中。在第 2 组中，将 0.2 毫升 VX2 癌切碎的小肿瘤碎片悬浮液插入 18 号针中，植入肝实质中。使用计算机断层扫描（CT）扫描、血管造影和病理标本评估和比较肿瘤存活率、大小和肝外转移。植入后 2 周，第 1 组 22 只兔子中的 14 只（63.6%）和第 2 组 22 只兔子中的 21 只（95.5%）成功实现了肝脏肿瘤生长（P
The rabbit VX2 hepatic tumor model has been widely used for the investigation of liver cancer. The present study was designed to compare two inoculation methods in order to identify an improved modality for establishing rabbit hepatic tumors. A total of 44 rabbits were randomly divided into 2 groups. All inoculations were performed by open laparotomy. In group 1,22 rabbits received a VX2 tumor cell suspension in a volume of 0.2 ml, which was slowly injected into the rabbit liver parenchyma using an 18-gauge needle. In group 2, a 0.2-ml suspension of small, minced tumor fragments of a VX2 carcinoma, inserted into an 18-gauge needle, was implanted into the liver parenchyma. The tumor survival ratio, size and extrahepatic metastases were evaluated and compared using computed tomography (CT)-scan, angiography and pathological specimens. At 2 weeks following implantation, successful liver tumor growth was achieved in 14 out of the 22 rabbits in group 1 (63.6%) and 21 out of the 22 rabbits in group 2 (95.5%) (P