Ultrasensitive fluorescence detection of heparin based on quantum dots and a functional ruthenium polypyridyl complex
Ultrasensitive fluorescence detection of heparin based on quantum dots and a functional ruthenium polypyridyl complex
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基于量子点和功能性钌聚吡啶复合物的肝素超灵敏荧光检测
DOI：
10.1016/j.bios.2013.12.009
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发表时间：
2014-05-15
影响因子：
12.6
通讯作者：
Yao, Tianming
中科院分区：
文献类型：
--
作者：
Cao, Yanlin;Shi, Shuo;Yao, Tianming
利用量子点（QDs）和钌多吡啶配合物[Ru（phen）（2）（dppz-idzo）]（2+）（phen= 1，10-菲咯啉，dppz-idzo =联吡啶并[3，2-a：2 '，3'-c]吩嗪-咪唑啉酮）建立了一种新的检测肝素的方法。发现Ru配合物由于电子转移而使CdTe量子点的发射猝灭。加入聚阴离子肝素后，由于肝素与Ru络合物之间的静电和/或氢键作用，可以将猝灭剂（Ru络合物）从量子点表面去除，从而导致CdTe量子点的荧光恢复。肝素在缓冲溶液中的荧光强度随肝素浓度的增加而增强，线性范围为21-77 nM，检测限为0.38 nM。此外，该策略成功地应用于检测低至0.68 nM的肝素，线性范围为35-98 nM的胎牛血清样品。荧光分析的选择性结果表明，我们的系统显示出优异的荧光选择性肝素超过其类似物，如软骨素4-硫酸酯（Chs）或透明质酸（Hya）。该方法无需标记，操作简便，灵敏度高，选择性好，可用于生物系统中肝素的检测。(C)2013 Elsevier B. V.保留所有权利。
A new strategy for the detection of heparin is developed by utilizing quantum dots (QDs) and a functional ruthenium polypyridyl complex [Ru(phen)(2)(dppz-idzo)](2+) (phen=1,10-phenanthroline, dppz-idzo = dipyrido-[3,2-a:2',3'-c] phenazine-imidazolone). The emission of CdTe QDs is found to be quenched by Ru complex due to electron transfer. Upon addition of the polyanionic heparin, it could remove the quencher (Ru complex) from the surface of QDs owing to the electrostatic and/or hydrogen bonding interactions between heparin and Ru complex, which led to significant fluorescence recovery of CdTe QDs. The fluorescence intensity enhanced with the increase of heparin and a linear relationship was observed in the range of 21-77 nM for heparin detection in buffer solution and the limit of detection (LOD) is 0.38 nM. Moreover, the strategy was successfully applied to detect heparin as low as 0.68 nM with a linear range of 35-98 nM in fetal bovine serum samples. The selectivity results of the fluorescence assay revealed that our system displayed excellent fluorescence selectivity towards heparin over its analogues, such as chondroitin 4-sulfate (Chs) or hyaluronic acid (Hya). This fluorescence "switch on" assay for heparin is label-free, convenient, sensitive and selective, which can be used to detect heparin in biological systems even with the naked eyes. (C) 2013 Elsevier B.V. All rights reserved.