Interspecific Hybrids between Iris setosa var. setosa and I. laevigata and Their Relationships to I. setosa var. hondoensis or I. setosa var. nasuensis
Interspecific Hybrids between Iris setosa var. setosa and I. laevigata and Their Relationships to I. setosa var. hondoensis or I. setosa var. nasuensis
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Iris setosa var. 之间的种间杂交。
DOI：
10.1508/cytologia.73.401
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发表时间：
2008
期刊：
影响因子：
1
通讯作者：
T. Yabuya
中科院分区：
文献类型：
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作者：
Kouichi Inoue;Takeshi Tomita;N. Yoshihara;T. Yabuya
刚毛鸢尾hondoensis和I.塞托萨湾nasuensis是日本特有的，它们的起源仍然有待澄清。在这项研究中，我们报告了同源四倍体I。塞托萨湾setosa（4x）和二倍体I. laevigata（2n=32）及其与I.塞托萨湾hondoensis或I.塞托萨湾那须。在相互杂交中，I.塞托萨湾setosa（2x，4x）和I. laevigata杂交获得的后代植株中，只有I.塞托萨湾setosa（4x）和I. laevigata（2x）.经鉴定，这些后代分别为异源三倍体（2n=54）和非整倍体（2n=53）。塞托萨湾setosa（4x）×I.通过流式细胞仪（FCM）、细胞学和随机扩增多态性DNA（RAPD）分析，对10个杂交种（SL 1 -5）进行了鉴定。此外，I.塞托萨湾hondoensis和I.塞托萨湾nasuensis与SL 1 -5进行了比较。对这些品种进行了FCM、细胞学、RAPD和CAPS分析，比较分析表明，这些品种具有I.塞托萨湾setosa和我laevigata和母本植物是前者的细胞器DNA。
Iris setosa var. hondoensis and I. setosa var. nasuensis are endemic to Japan, and their origins still remain to be clarified. In this study, we report the characterization of interspecific hybrids (allotriploids) from the cross between autotetraploid I. setosa var. setosa (4x) and diploid I. laevigata (2n=32) and their relationship to I. setosa var. hondoensis or I. setosa var. nasuensis. In reciprocal crosses between I. setosa var. setosa (2x, 4x) and I. laevigata, progeny plants were obtained only in the cross between I. setosa var. setosa (4x) and I. laevigata (2x). These progenies were identified as allotriploids (2n=54) and their aneuploids (2n=53) of I. setosa var. setosa (4x)×I. laevigata (2x) by flow cytometric (FCM), cytological and random amplified polymorphic DNA (RAPD) analyses, and the five hybrids (SL1–5) except SL6 were characterized. Furthermore, these characteristics of I. setosa var. hondoensis and I. setosa var. nasuensis were compared with those of SL1–5. These varieties were also examined by FCM, cytological, RAPD and cleaved amplified polymorphic sequence (CAPS) analyses, which comparative analyses showed that varieties have the hybrid nature between I. setosa var. setosa and I. laevigata, and maternal plants are organelle DNAs of the former species.
通过鸢尾原生质体培养高效生产多倍体植物。
DOI：
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发表时间：
2004
期刊：
Cytologia 69・3
影响因子：
--
作者：
Inoue;K.;T.Kato;H.Kunitake;T.Yabuya
通讯作者：
T.Yabuya