Modulation of neutrophil motility by curcumin: implications for inflammatory bowel disease.
Modulation of neutrophil motility by curcumin: implications for inflammatory bowel disease.
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DOI：
10.1002/ibd.21391
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发表时间：
2011-02
影响因子：
4.9
通讯作者：
Kiela, P. R.
中科院分区：
文献类型：
--
作者：
Larmonier, C. B.;Midura-Kiela, M. T.;Ramalingam, R.;Laubitz, D.;Janikashvili, N.;Larmonier, N.;Ghishan, F. K.;Kiela, P. R.
Neutrophils (PMN) are the first cells recruited at the site of inflammation. They play a key role in the innate immune response by recognizing, ingesting and eliminating pathogens and participate in the orientation of the adaptive immune responses. However, in Inflammatory Bowel Disease (IBD), transepithelial neutrophil migration leads to an impaired epithelial barrier function, perpetuation of inflammation and tissue destruction via oxidative and proteolytic damage. Curcumin (diferulolylmethane) displays a protective role in mouse models of IBD and in human ulcerative colitis, a phenomenon consistently accompanied by a reduced mucosal neutrophil infiltration. We investigated the effect of curcumin on mouse and human neutrophil polarization and motility in vitro and in vivo. Curcumin attenuated LPS-stimulated expression and secretion of MIP-2, IL-1β, KC and MIP-1α in colonic epithelial cells (CEC) and in macrophages. Curcumin significantly inhibited PMN chemotaxis against MIP-2, KC or against conditioned media from LPS-treated macrophages or CEC, a well as the IL-8-mediated chemotaxis of human neutrophils. At non-toxic concentrations, curcumin inhibited random neutrophil migration suggesting a direct effect on neutrophil chemokinesis. Curcumin-mediated inhibition of PMN motility could be attributed to a downregulation of PI3K activity, AKT phosphorylation and F-actin polymerization at the leading edge. The inhibitory effect of curcumin on neutrophil motility was further demonstrated in vivo in a model of aseptic peritonitis. Our results indicate that curcumin interferes with colonic inflammation partly through inhibition of the chemokine expression and through direct inhibition of neutrophil chemotaxis and chemokinesis.
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DOI：
10.1124/jpet.108.145862
发表时间：
2009-04-01
影响因子：
3.5
作者：
Farooq, Shukkur Muhammed;Stillie, RoseMarie;Stadnyk, Andrew W.
通讯作者：
Stadnyk, Andrew W.
影响因子：
3.1
作者：
D'Odorico, A;D'Inca, R;Sturniolo, GC
通讯作者：
Sturniolo, GC
影响因子：
1.2
作者：
Anezaki, K;Asakura, H;Narisawa, R
通讯作者：
Narisawa, R
影响因子：
4.9
作者：
Billerey-Larmonier, Claire;Uno, Jennifer K.;Kiela, Pawel R.
通讯作者：
Kiela, Pawel R.
影响因子：
12.6
作者：
Hanai, Hiroyuki;Iida, Takayuki;Koide, Yukio
通讯作者：
Koide, Yukio