Human polyomavirus BK (BKV) load and haemorrhagic cystitis in bone marrow transplantation patients
Human polyomavirus BK (BKV) load and haemorrhagic cystitis in bone marrow transplantation patients
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DOI：
10.1016/s1386-6532(99)00055-4
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发表时间：
1999-10-01
影响因子：
8.8
通讯作者：
Bosi, A
中科院分区：
文献类型：
--
作者：
Azzi, A;Cesaro, S;Bosi, A
一些观察结果表明，骨髓移植（BMT）受者的长期出血性膀胱炎（HC）与人多瘤病毒BK （BKV）再激活之间存在关联，但迄今尚未获得确凿证据。为了更好地评估BKV再激活与HC-的关系，我们将HC发作期间BMT患者尿液中BKV的检测值与未HC的BMT患者和免疫正常个体尿液中BKV的检测值进行了比较。为此目的，开发了一种定量竞争性PCR。将该检测应用于临床样本，使我们能够在几乎所有病例中区分健康个体和免疫功能低下患者的无症状再激活与HC相关的再激活。在无症状的健康个体和大约50%的免疫功能低下患者中，显示出低于定量检测灵敏度的低水平。在HC患者的尿液中检测到明显高于无症状免疫功能低下患者尿液中的病毒载量。这些数据加强了BKV再激活可与其他因素一起导致BMT受体和急性难治性淋巴细胞白血病患者中大多数晚期HC的假设。(C) 1999 Elsevier Science B.V.版权所有
Several observations suggest an association between long-lasting haemorrhagic cystitis (HC) in bone marrow transplantation (BMT) recipients and human polyomavirus BK (BKV) reactivation, but no conclusive evidence has been obtained so far. The amount of BKV measured in the urine of BMT patients during an episode of HC was compared with that detected in the urine of BMT patients without HC and of immunocompetent individuals in order to better assess the association of BKV reactivation with HC-. For this purpose a quantitative competitive PCR was developed. The application of this assay to clinical samples allowed us to distinguish asymptomatic reactivation both in healthy individuals and in immunocompromised patients from reactivation associated with HC, in almost all cases. Low levels, below the sensitivity of the quantitative assay, were shown in asymptomatic healthy individuals and in about 50% of immunocompromised patients. A significantly higher viral load than in the urine of asymptomatic immunocompromised patients was detected in the urine of patients with HC. These data strengthen the hypothesis that BKV reactivation can cause, together with other factors, the majority of late HC in BMT recipients as well as in patients treated for acute refractory lymphoblastic leukemia. (C) 1999 Elsevier Science B.V. All rights reserved.