FRACTIONATION OF FOWL IMMUNOGLOBULINS
FRACTIONATION OF FOWL IMMUNOGLOBULINS
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DOI：
10.1016/s0034-5288(18)33400-3
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发表时间：
1976-01-01
影响因子：
2.4
通讯作者：
HIGGINS, DA
中科院分区：
文献类型：
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作者：
HIGGINS, DA
用Sephadex G-200凝胶过滤、DEAE Sephadex A-50离子交换层析和10-40%蔗糖梯度超速离心法分离12周龄鸡血清、Na2SO4沉淀的血清免疫球蛋白和胆汁，以及1日龄鸡血清。免疫球蛋白、免疫球蛋白、免疫球蛋白和白蛋白的洗脱是通过检测琼脂扩散中抗这些蛋白的抗血清的组分来监测的。12周龄鸡血清和胆汁中含有2种分子大小的IgM和1种分子大小的Ig G。1日龄的鸡血清含有估计为7S的IgM，除了正常的7S成分外，还有一种聚合形式的免疫球蛋白，以及一种与IgA抗原相关的小分子蛋白。胆汁中大部分白蛋白的相对分子质量低于血清白蛋白，而在超速离心胆汁和雏鸡血清中可检测到较重的白蛋白。
Serum, Na2SO4-precipitated serum immunoglobulins [Ig] and bile from 12 wk old fowl [chickens], and serum from day-old chickens, were fractionated by Sephadex G-200 gel filtration, DEAE Sephadex A-50 ion exchange chromatography and ultracentrifugation through 10-40% sucrose gradients. Elution of IgM, IgG, IgA and albumin was monitored by examination of fractions in agar gel diffusion against [rabbit] antisera specific to these proteins. Serum and bile from 12 wk old fowl contained IgM and IgA in 2 molecular sizes and a single molecular size of IgG. Day-old chick serum contained IgM estimated to be 7S, a polymerized form of IgG in addition to the normal 7S component, and a small MW protein antigenically related to IgA. Most of the albumin in bile was of lower MW than serum albumin, while heavy forms of albumin were detected in ultracentrifugation of bile and day-old chick serum.