Clinical Significance of a High SARS-CoV-2 Viral Load in the Saliva
Clinical Significance of a High SARS-CoV-2 Viral Load in the Saliva
复制标题
DOI：
10.3346/jkms.2020.35.e195
复制
发表时间：
2020-05-25
影响因子：
4.5
通讯作者：
Kim, Woo Joo
中科院分区：
文献类型：
--
作者：
Yoon, Jin Gu;Yoon, Jung;Kim, Woo Joo
背景资料：2019年冠状病毒病（COVID-19）患者在早期亚临床阶段可以在不知不觉中将病毒传播给几个人。方法：我们评估了两名COVID-19患者从住院第1天到第9天的各种体液标本中的病毒动态，如鼻咽拭子，口咽拭子，唾液，痰和尿液标本。在使用洗必泰漱口水后1小时、2小时和4小时采集额外的唾液样本。采用实时荧光定量逆转录聚合酶链反应（real-time reverse transcriptase polymerase chain reaction，rRT-PCR）检测2例患者5份标本中SARS冠状病毒2型（SARS CoV-2）病毒载量。鼻咽中的病毒载量最高（患者1= 8.41 log（10）拷贝/mL;患者2 = 7.49 log（10）拷贝/mL），但唾液中的病毒载量也非常高（患者1= 6.63 log in copies/mL;患者2 = 7.10 log in copies/mL）。SARS-CoV-2在两名患者的唾液中被检测到，直到住院第6天（患者2的患病第9天）。结论：SARS-CoV-2病毒载量在COVID-19早期唾液中持续高水平，且相对高于口咽部。洗必泰漱口水可在短期内有效降低唾液中的SARS-CoV-2病毒载量。
Background: Patients with coronavirus disease 2019 (COVID-19) can unknowingly spread the virus to several people during the early subclinical period.Methods: We evaluated the viral dynamics in various body fluid specimens, such as nasopharyngeal swab, oropharyngeal swab, saliva, sputum, and urine specimens, of two patients with COVID-19 from hospital day 1 to 9. Additional samples of the saliva were taken at 1 hour, 2 hours, and 4 hours after using a chlorhexidine mouthwash. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral load was determined by real-time reverse transcriptase polymerase chain reaction (rRT-PCR).Results: SARS-CoV-2 was detected from all the five specimens of both patients by rRT-PCR. The viral load was the highest in the nasopharynx (patient 1= 8.41log(10) copies/mL; patient 2 = 7.49 log(10) copies/mL), but it was also remarkably high in the saliva (patient 1= 6.63 log in copies/mL; patient 2 = 7.10 log in copies/mL). SARS-CoV-2 was detected up to hospital day 6 (illness day 9 for patient 2) from the saliva of both patients. The viral load in the saliva decreased transiently for 2 hours after using the chlorhexidine mouthwash.Conclusion: SARS-CoV-2 viral load was consistently high in the saliva; it was relatively higher than that in the oropharynx during the early stage of COVID-19. Chlorhexidine mouthwash was effective in reducing the SARS-CoV-2 viral load in the saliva for a short-term period.