Gentamicin is primarily localized in vestibular type I hair cells after intratympanic administration
Gentamicin is primarily localized in vestibular type I hair cells after intratympanic administration
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DOI：
10.1007/s10162-007-0093-8
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发表时间：
2007-12-01
影响因子：
2.4
通讯作者：
Carey, John P.
中科院分区：
文献类型：
--
作者：
Lyford-Pike, Sofia;Vogelheim, Casey;Carey, John P.
鼓室内庆大霉素注射是有效的控制发作性眩晕，由于梅尼耶病。动物组织学研究发现，IT庆大霉素治疗后，I型前庭毛细胞的损失远远超过11型细胞的损失。本研究的目的是确定这种对I型毛细胞的选择性毒性是否可能是由于这些细胞对药物的选择性浓度。庆大霉素可通过直接和间接方法定位于前庭上皮内。庆大霉素偶联Texas Redo被用作直接示踪剂，而抗庆大霉素抗体提供了间接的定位手段。将偶联或未偶联庆大霉素注入龙猫左鼓室。治疗后1 ~ 3周处死固定。用共聚焦荧光显微镜测定庆大霉素在半规管嵴中的定位。在给药一周内死亡的动物的结果显示，许多I型毛细胞仍然遍布上皮。I型毛细胞抗庆大霉素标记的平均灰度单位（0-255）为28.14 (95% Cl 24.60-31.69)， 11型毛细胞为17.09(14.99-19.20)，支持细胞为5.35 （5.345.46）
Intratympanic (IT) gentamicin injections are effective in the control of episodic vertigo due to Menier's disease. Histological studies in animals have found that the loss of type I vestibular hair cells far exceeds that of type 11 cells after IT gentamicin treatment. The objective of this study was to determine whether this selective toxicity for type I hair cells might be due to selective concentration of the drug by these cells. Gentamicin was localized within the vestibular epithelium by both direct and indirect methods. Gentamicin conjugated to Texas Redo was used as a direct tracer, and anti-gentamicin antibody provided an indirect means of localization. Conjugated or unconjugated gentamicin was injected into the left tympanic space of chinchillas. The animals were killed and fixed I or 3 weeks post-treatment. Confocal fluorescence microscopy was used to determine the localization of gentamicin in semicircular canal cristae. Results from the animals killed within I week of administration showed that numerous type I hair cells still remained throughout the epithelium. The mean intensity in grayscale units (0-255) of anti-gentamicin labeling for type I hair cells was 28.14 (95% Cl 24.60-31.69), for type 11 hair cells was 17.09 (14.99-19.20), and for support cells was 5.35 (5.345.46; p