The proteins of biologically active sub-units of vesicular stomatitis virus.
The proteins of biologically active sub-units of vesicular stomatitis virus.
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水泡性口炎病毒生物活性亚基的蛋白质。
DOI：
10.1099/0022-1317-7-3-267
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发表时间：
1970
期刊：
影响因子：
--
通讯作者：
F. Brown
中科院分区：
文献类型：
--
作者：
B. Cartwright;P. Talbot;F. Brown
我们对水泡性口炎病毒逐步降解成具有良好特征的结构成分的实验使我们能够将病毒的一些生物学特性与其某些结构单元联系起来(Cartwright，Smer&Brown，1969,1970)。例如，用胰酶孵育病毒会产生类似于病毒的子弹状结构，只是它不再具有表面突起。与病毒不同的是，无突起成分的传染性较低，当接种到豚鼠体内时不会产生中和抗体，从而提供了决定性的证据，表明病毒的免疫活性与表面突起有关。通过用吐温+乙醚或诺尼特P40或脱氧胆酸钠治疗病毒(Brown，Cartwright&Smer，1967；Cartwright等人)。1970年)，免疫抗原可以以生物活性的形式释放，以大约6秒的速度沉淀。用吐温+乙醚或诺奈特处理病毒也会产生与病毒大小和形状相同的感染性骨架状结构。
Our experiments on the stepwise degradation of vesicular stomatitis virus into well characterized structural components have enabled us to relate some of the biological properties of the virus to certain of its structural units (Cartwright, Smale & Brown, 1969, 1970). For example, incubating the virus with trypsin produces a bullet-shaped structure similar to the virus except that it no longer possesses the surface projections. Unlike the virus, the projection-free component has low infectivity and does not produce neutralizing antibodies when inoculated into guinea-pigs, thus providing decisive evidence that the immunizing activity of the virus is associated with the surface projections. By treating the virus with Tween + ether or Nonidet P40 or sodium deoxycholate (Brown, Cartwright & Smale, 1967; Cartwright et al. 1970), the immunizing antigen can be released in a biologically active form, sedimenting at about 6s. An infective skeleton-like structure with the same size and shape as the virus is also produced by treating the virus with Tween + ether or with Nonidet.