EFFECT OF PH ON COLLAGEN FLEXIBILITY DETERMINED FROM DILUTE-SOLUTION VISCOELASTIC MEASUREMENTS
EFFECT OF PH ON COLLAGEN FLEXIBILITY DETERMINED FROM DILUTE-SOLUTION VISCOELASTIC MEASUREMENTS
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DOI：
10.1016/0141-8130(85)90014-5
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发表时间：
1985-01-01
影响因子：
8.2
通讯作者：
VEIS, A
中科院分区：
文献类型：
--
作者：
AMIS, EJ;CARRIERE, CJ;VEIS, A
The frequency dependences of the storage and loss shear moduli, G'' and G'''', of dilute solutions of collagen at pH 7.4, ionic strength approximately 0.2, were measured at 15.0.degree. C by the Birnboim-Schrag multiple-lumped resonator apparatus. By use of 2 solvents, water and 50% glycerol, the effective reduced frequency range was extended to cover 2.5 logarithmic decades. The intrinsic viscosity and longest (rotational) relaxation time were considerably smaller than those determined at pH 4.0 in an earlier study. At pH 4.0, the behavior could be modelled by a rodlike molecule with partial flexibility along its entire length and a persistence length of 161 nm with no loose joints. The behavior at pH 7.4 corresponds approximately to the expectation for a semiflexible rod with 2 loose joints near the ends and a similar persistence length (169 nm) for the center segment.
