PROTEIN-TURNOVER MEASURED INVIVO AND INVITRO IN MUSCLES UNDERGOING COMPENSATORY GROWTH AND SUBSEQUENT DENERVATION ATROPHY
PROTEIN-TURNOVER MEASURED INVIVO AND INVITRO IN MUSCLES UNDERGOING COMPENSATORY GROWTH AND SUBSEQUENT DENERVATION ATROPHY
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DOI：
10.1042/bj2100089
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发表时间：
1983-01-01
影响因子：
4.1
通讯作者：
MCNURLAN, MA
中科院分区：
文献类型：
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作者：
GOLDSPINK, DF;GARLICK, PJ;MCNURLAN, MA
功能超负荷的[兔]比目鱼肌因协同腓肠肌腱切断术而快速生长（1-6天），与蛋白质合成率和降解率的增加相关，前者的变化大于后者。这些结论是通过两种不同的方法得出的，这些方法用于测量（体内和体外）这些比目鱼肌中蛋白质合成和蛋白质分解的平均速率。尽管体内测量时的基础合成率较高，而体外培养的离体肌肉制剂的降解率较高，但两种方法对于腓肠肌腱切断术引起的蛋白质周转变化具有良好的一致性。讨论了被动拉伸可能参与诱导这种额外生长的可能性。作为比目鱼肌的拮抗剂，在相同条件下，趾长伸肌的生长减少，可能是因为使用较少。三天后，坐骨神经被切断后，之前正常或超负荷的比目鱼肌迅速萎缩。尽管在这两种情况下，RNA和蛋白质都丢失了，同时蛋白质合成减少了，蛋白质分解增加了，但去神经支配导致了先前超负荷肌肉的这些参数发生了更大的变化。腱切断腓肠肌的生长减慢及其在额外去神经后随后的快速萎缩，可以通过蛋白质分解的大幅增加来解释，而合成率几乎没有变化或没有变化。
The rapid growth (1-6 days) of the functionally overloaded [rabbit] soleus muscle, in response to tenotomy of the synergist gastrocnemius, correlated with increases in both the protein synthetic and degradative rates, the change in the former being greater than that of the latter. These conclusions were drawn from 2 different methods used to measure (in vivo and in vitro) the average rates of protein synthesis and protein breakdown in these soleus muscles. Although the basal rates of synthesis were higher when measured in vivo and the degradative rates higher in isolated muscle preoparations incubated in vitro, both methods gave good agreement concerning the changes in protein turnover induced by tenotomy of the gastrocnemius. The possible involvement of passive stretch in inducing this additional growth is discussed. As an antagonist to the soleus, growth of the extensor digitorum longus muscle was decreased under the same conditions, presumably because of less usage. At 3 days, after the cutting of the sciatic nerve, the previously normal or overloaded soleus muscles underwent rapid atrophy. Although in both cases RNA and protein were lost, while protein synthesis decreased and protein breakdown increased, denervation induced larger changes within these parameters of the formerly overloaded muscle. The slowing of growth in the tenotomized gastrocnemius and its subsequent rapid atrophy after additional denervation, were explained by large increases in protein breakdown, with little or no change in the synthetic rate.