Rejuvenation of antioxidant system in central nervous system of aged rats by grape seed extract
Rejuvenation of antioxidant system in central nervous system of aged rats by grape seed extract
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DOI：
10.1016/j.neulet.2005.04.042
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发表时间：
2005-08-05
影响因子：
2.5
通讯作者：
Panneerselvam, C
中科院分区：
文献类型：
--
作者：
Balu, M;Sangeetha, P;Panneerselvam, C
氧化应激被认为是导致衰老相关的脂质过氧化增加和中枢神经系统中抗氧化剂在衰老过程中下降的主要危险因素。葡萄籽提取物是生物黄酮类化合物的一种，因其药用价值而被广泛应用。在本研究中，我们评估了葡萄籽提取物对幼年和老年大鼠中枢神经系统不同区域的脂质过氧化和抗氧化状态的作用。将Wistar系雄性白化大鼠分为4组：I组-对照组幼鼠，II组-幼年大鼠给予葡萄籽提取物(100 mg/kg体重)30d，III组-老年对照组大鼠和IV组-老年大鼠补充葡萄籽提取物(100 mg/kg体重)30d。老龄大鼠脊髓、大脑皮层、纹状体和海马区的脂质过氧化程度随年龄增长而增加(第111组)。研究发现，与年轻大鼠相比，老年大鼠所有大脑区域的超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活性以及还原型谷胱甘肽、维生素C和维生素E等非酶类抗氧化剂的水平都显著下降。然而，在补充葡萄籽提取物的老年大鼠中，报告了正常化的脂质过氧化和抗氧化防御。这些结果表明葡萄籽提取物提高了老龄大鼠中枢神经系统的抗氧化状态，降低了自由基诱导的中枢神经系统脂质过氧化的发生率。(C)2005爱思唯尔爱尔兰有限公司。保留所有权利。
Oxidative stress is considered as a major risk factor that contributes to age-related increase in lipid peroxidation and declined antioxidants in the central nervous system during aging. Grape seed extract, one of the bioflavonoid, is widely used for its medicinal properties. In the present study, we evaluated the role of grape seed extract on lipid peroxidation and antioxidant status in discrete regions of the central nervous system of young and aged rats. Male albino rats of Wistar strain were divided into four groups: Group I-control young rats, Group II-young rats treated with grape seed extract (100 mg/kg body weight) for 30 days, Group III-aged control rats and Group IV-aged rats supplemented with grape seed extract (100 mg/kg body weight) for 30 days. Age-associated increase in lipid peroxidation was observed in the spinal cord, cerebral cortex, striatum and the hippocampus regions of aged rats (Group 111). Activities of antioxidant enzymes like superoxide dismutase, catalase, glutathione peroxidase and levels of non-enzymic antioxidants like reduced glutathione, Vitamin C and Vitamin E were found to be significantly decreased in all the brain regions studied in aged rats when compared to young rats. However, normalized lipid peroxidation and antioxidant defenses were reported in the grape seed extract-supplemented aged rats. These findings demonstrated that grape seed extract enhanced the antioxidant status and decreased the incidence of free radical-induced lipid peroxidation in the central nervous system of aged rats. (c) 2005 Elsevier Ireland Ltd. All rights reserved.